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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GMIP Polyclonal antibody (25917-1-AP)

    WB analysis using 25917-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25917-1-AP (GMIP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of Jurkat cells using GMIP Polyclonal antibody (25917-1-AP)

    IP experiment of Jurkat using 25917-1-AP

    IP result of anti-GMIP (IP:25917-1-AP, 4ug; Detection:25917-1-AP 1:500) with Jurkat cells lysate 4000ug.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using GMIP Polyclonal antibody (25917-1-AP)

    IHC staining of human stomach cancer using 25917-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 25917-1-AP (GMIP antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using GMIP Polyclonal antibody (25917-1-AP)

    IHC staining of human ovary cancer using 25917-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 25917-1-AP (GMIP antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using GMIP Polyclonal antibody (25917-1-AP)

    IF Staining of HepG2 using 25917-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using GMIP antibody (25917-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using GMIP Polyclonal antibody (25917-1-AP)

    IF Staining of HepG2 using 25917-1-AP

    Immunofluorescent analysis of HepG2 cells using 25917-1-AP (GMIP antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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GMIP Polyclonal antibody

GMIP Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 25917-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

GEM-interacting protein, GEM interacting protein, ARHGAP46

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inJurkat cells, K-562 cells
Positive IP detected inJurkat cells
Positive IHC detected inhuman stomach cancer tissue, human ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25917-1-AP targets GMIP in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GMIP fusion protein Ag23239 相同性解析による交差性が予測される生物種
Full Name GEM interacting protein
Observed molecular weight 130 kDa
GenBank accession numberBC126436
Gene Symbol GMIP
Gene ID (NCBI) 51291
RRIDAB_2880295
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9P107
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GMIP (Gem-interacting protein) is a 970 amino acid protein that stimulates the GTPase activity of RhoA in vitro and in vivo. GMIP interacts with Gem through its N-terminus and has a Rho GTPase-activating protein domain at its C-terminus. The Rho family of GTP-binding proteins plays a role in the development of neuronal structure. GMIP is able to inhibit RhoA function, leading to Actin cytoskeletal reorganization in vivo.

Protocols

Product Specific Protocols
WB protocol for GMIP antibody 25917-1-APDownload protocol
IHC protocol for GMIP antibody 25917-1-APDownload protocol
IF protocol for GMIP antibody 25917-1-APDownload protocol
IP protocol for GMIP antibody 25917-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

Int Immunopharmacol

Identification of TAP1 as a T-cell related therapeutic target in gastric cancer by mediating oxalipliatin-related synergistic enhancement of immunotherapy

Authors - Yupeng Zhao
View Article