GMPS Polyclonal antibody

GMPS Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Cat no : 16376-1-AP

Synonyms

Glutamine amidotransferase, GMP synthetase, GMPS


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Tested Applications

Positive WB detected inHeLa cells, HL-60 cells, Jurkat cells, mouse brain tissue, mouse testis tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16376-1-AP targets GMPS in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GMPS fusion protein Ag9350 相同性解析による交差性が予測される生物種
Full Name guanine monphosphate synthetase
Calculated molecular weight 693 aa, 77 kDa
Observed molecular weight 67 kDa
GenBank accession numberBC012178
Gene symbol GMPS
Gene ID (NCBI) 8833
RRIDAB_2232412
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for GMPS antibody 16376-1-APDownload protocol
IHC protocol for GMPS antibody 16376-1-APDownload protocol
IP protocol for GMPS antibody 16376-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Mol Cell

ERK2 Phosphorylates PFAS to Mediate Posttranslational Control of De Novo Purine Synthesis.

Authors - Eunus S Ali
humanWB, IHC

PLoS Biol

Nucleotide de novo synthesis increases breast cancer stemness and metastasis via cGMP-PKG-MAPK signaling pathway.

Authors - Yajing Lv
humanWB

Aging (Albany NY)

The upregulated expression of RFC4 and GMPS mediated by DNA copy number alteration is associated with the early diagnosis and immune escape of ESCC based on a bioinformatic analysis.

Authors - Jing Wang
humanWB,IHC

J Biomed Sci

TRIM21-SERPINB5 aids GMPS repression to protect nasopharyngeal carcinoma cells from radiation-induced apoptosis.

Authors - Panpan Zhang
mouseWB

Cell Rep

Mutant CHCHD10 causes an extensive metabolic rewiring that precedes OXPHOS dysfunction in a murine model of mitochondrial cardiomyopathy.

Authors - Nicole M Sayles
human,mouseWB,IHC

J Proteome Res

Comprehensive Urinary Proteome Profiling Analysis Identifies Diagnosis and Relapse Surveillance Biomarkers for Bladder Cancer

Authors - Qi Chang