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Species Reactivity

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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse lung tissue using GNA14 Polyclonal antibody (13350-1-AP)

    WB analysis of mouse lung using 13350-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 13350-1-AP (GNA14 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse lung tissue using GNA14 Polyclonal antibody (13350-1-AP)

    WB analysis of mouse lung using 13350-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 13350-1-AP (GNA14 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human kidney tissue using GNA14 Polyclonal antibody (13350-1-AP)

    IHC staining of human kidney using 13350-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 13350-1-AP (GNA14 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using GNA14 Polyclonal antibody (13350-1-AP)

    IHC staining of human kidney using 13350-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 13350-1-AP (GNA14 Antibody) at dilution of 1:200 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using GNA14 Polyclonal antibody (13350-1-AP)

    IF Staining of HeLa using 13350-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using GNA14 antibody (13350-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using GNA14 Polyclonal antibody (13350-1-AP)

    IF Staining of HeLa using 13350-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 13350-1-AP (GNA14 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using GNA14 Polyclonal antibody (13350-1-AP)

    IF Staining of HepG2 using 13350-1-AP

    Immunofluorescent analysis of HepG2 cells, using GNA14 antibody 13350-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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GNA14 Polyclonal antibody

GNA14 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 13350-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Guanine nucleotide-binding protein subunit alpha-14, G-protein subunit alpha-14, G alpha-14

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse lung tissue
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells, HepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13350-1-AP targets GNA14 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GNA14 fusion protein Ag3930 相同性解析による交差性が予測される生物種
Full Name guanine nucleotide binding protein (G protein), alpha 14
Calculated molecular weight 355 aa, 42 kDa
Observed molecular weight 42 kDa
GenBank accession numberBC027886
Gene Symbol GNA14
Gene ID (NCBI) 9630
RRIDAB_2877942
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO95837
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for GNA14 antibody 13350-1-APDownload protocol
IHC protocol for GNA14 antibody 13350-1-APDownload protocol
IF protocol for GNA14 antibody 13350-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols