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Target

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Species Reactivity

Species Reactivity

Conjugate

Conjugate

GNAI1 Polyclonal antibody

GNAI1 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12617-1-AP

Synonyms

Gi, GNAI1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GNAI1 Polyclonal antibody (12617-1-AP)

    WB analysis using 12617-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12617-1-AP (GNAI1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of fetal human brain tissue using GNAI1 Polyclonal antibody (12617-1-AP)

    WB analysis of fetal human brain using 12617-1-AP

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 12617-1-AP (GNAI1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of SH-SY5Y cells using GNAI1 Polyclonal antibody (12617-1-AP)

    IP experiment of SH-SY5Y using 12617-1-AP

    IP result of anti-GNAI1 (IP:12617-1-AP, 4ug; Detection:12617-1-AP 1:1000) with SH-SY5Y cells lysate 800 ug.

  • Immunohistochemistry (IHC) staining of human kidney tissue using GNAI1 Polyclonal antibody (12617-1-AP)

    IHC staining of human kidney using 12617-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12617-1-AP (GNAI1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using GNAI1 Polyclonal antibody (12617-1-AP)

    IHC staining of human kidney using 12617-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12617-1-AP (GNAI1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat kidney tissue using GNAI1 Polyclonal antibody (12617-1-AP)

    IHC staining of rat kidney using 12617-1-AP

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 12617-1-AP (GNAI1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat kidney tissue using GNAI1 Polyclonal antibody (12617-1-AP)

    IHC staining of rat kidney using 12617-1-AP

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 12617-1-AP (GNAI1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inmouse brain tissue, fetal human brain tissue, rat brain tissue
Positive IP detected inSH-SY5Y cells
Positive IHC detected inhuman kidney tissue, rat kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

12617-1-AP targets GNAI1 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GNAI1 fusion protein Ag3290 相同性解析による交差性が予測される生物種
Full Name guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 1
Calculated molecular weight 354 aa, 40 kDa
Observed molecular weight 38-40 kDa
GenBank accession numberBC026326
Gene symbol GNAI1
Gene ID (NCBI) 2770
RRIDAB_2111346
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The guanine nucleotide-binding protein G(i), α-1 subunit (GNAI1), also known as Gαi-1, belongs to the Gαi family. GNAI1 is an adenylate cyclase inhibitor that modulates cAMP. GNAI1 exerts inhibitory effects on cAMP production through its interaction with GPCR and the inactivation of adenyl cyclase (PMID: 35678269). GNAI1 has 2 isoforms with molecular weights of 35 and 40 kDa, respectively.

Protocols

Product Specific Protocols
WB protocol for GNAI1 antibody 12617-1-APDownload protocol
IHC protocol for GNAI1 antibody 12617-1-APDownload protocol
IP protocol for GNAI1 antibody 12617-1-APDownload protocol
FC protocol for GNAI1 antibody 12617-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Drug Des Devel Ther

Network Pharmacology and Experimental Verification to Explore the Potential Mechanism of Yin-Huo-Tang for Lung Adenocarcinoma Recurrence.

Authors - Dianna Liu
View Article
humanWB

J Oncol

Characterization of the Newly Established Homoharringtonine- (HHT-) Resistant Cell Lines and Mechanisms of Resistance

Authors - Fenglin Li
View Article