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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GNAI1 Monoclonal antibody (68266-1-Ig)

    WB analysis using 68266-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68266-1-Ig (GNAI1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of chicken brain tissue using GNAI1 Monoclonal antibody (68266-1-Ig)

    WB analysis of chicken brain using 68266-1-Ig

    chicken brain tissue were subjected to SDS PAGE followed by western blot with 68266-1-Ig (GNAI1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of pig brain tissue using GNAI1 Monoclonal antibody (68266-1-Ig)

    WB analysis of pig brain using 68266-1-Ig

    pig brain tissue were subjected to SDS PAGE followed by western blot with 68266-1-Ig (GNAI1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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GNAI1 Monoclonal antibody

GNAI1 Monoclonal Antibody for WB, ELISA
Cat No. 68266-1-Ig
Clone No.1G11H5

Host / Isotype

Mouse / IgG2b

Reactivity

Mouse, Rat, Pig, Chicken

Applications

WB, ELISA

Synonyms

Gi, GNAI1

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inrat brain tissue, pig brain tissue, chicken brain tissue, mouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68266-1-Ig targets GNAI1 in WB, ELISA applications and shows reactivity with Mouse, Rat, Pig, Chicken samples.

Tested Reactivity Mouse, Rat, Pig, Chicken
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen GNAI1 fusion protein Ag3290 相同性解析による交差性が予測される生物種
Full Name guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 1
Calculated molecular weight 354 aa, 40 kDa
Observed molecular weight 38-40 kDa
GenBank accession numberBC026326
Gene Symbol GNAI1
Gene ID (NCBI) 2770
RRIDAB_2935351
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP63096
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The guanine nucleotide-binding protein G(i), α-1 subunit (GNAI1), also known as Gαi-1, belongs to the Gαi family. GNAI1 is an adenylate cyclase inhibitor that modulates cAMP. GNAI1 exerts inhibitory effects on cAMP production through its interaction with GPCR and the inactivation of adenyl cyclase (PMID: 35678269). GNAI1 has 2 isoforms with molecular weights of 35 and 40 kDa, respectively.

Protocols

Product Specific Protocols
WB protocol for GNAI1 antibody 68266-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols