GNAI2 Polyclonal antibody

Name: GNAI2 Polyclonal antibody
Brand: Proteintech
SKU: 11136-1-AP

GNAI2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 11136-1-AP

Synonyms

H_LUCA16.1, H_LUCA15.1, Guanine nucleotide-binding protein G(i) subunit alpha-2, GNAI2B, GIP

Validation Data Gallery

Filter:

AllWBIPIHCIF/ICC

WB analysis using 11136-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11136-1-AP (GNAI2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis of fetal human brain using 11136-1-AP
fetal human brain tissue were subjected to SDS PAGE followed by western blot with 11136-1-AP (GNAI2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of SKOV-3 using 11136-1-AP
SKOV-3 cells were subjected to SDS PAGE followed by western blot with 11136-1-AP (GNAI2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of mouse brain using 11136-1-AP
IP result of anti-GNAI2 (IP:11136-1-AP, 4ug; Detection:11136-1-AP 1:500) with mouse brain tissue lysate 4000ug.
IHC staining of mouse brain using 11136-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11136-1-AP (GNAI2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 11136-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11136-1-AP (GNAI2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human ovary cancer using 11136-1-AP
Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 11136-1-AP (GNAI2 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human brain using 11136-1-AP
Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 11136-1-AP (GNAI2 Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human brain using 11136-1-AP
Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 11136-1-AP (GNAI2 Antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human lung cancer using 11136-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer using 11136-1-AP (GNAI2 antibody) at dilution of 1:10 (under 25x lens).
IHC staining of human tonsillitis using 11136-1-AP
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 11136-1-AP (GNAI2 Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human tonsillitis using 11136-1-AP
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 11136-1-AP (GNAI2 Antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human lung cancer using 11136-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer using 11136-1-AP (GNAI2 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of mouse ovary using 11136-1-AP
Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 11136-1-AP (GNAI2 antibody) at dilution of 1:50 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse ovary using 11136-1-AP
Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 11136-1-AP (GNAI2 antibody) at dilution of 1:50 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human kidney using 11136-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 11136-1-AP (GNAI2 Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human kidney using 11136-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 11136-1-AP (GNAI2 Antibody) at dilution of 1:50 (under 40x lens).
IF Staining of HeLa using 11136-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using GNAI2 antibody (11136-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Cat No. 11136-1-AP

Size/Concentration

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	fetal human brain tissue, mouse brain tissue, SKOV-3 cells, rat brain tissue
Positive IP detected in	mouse brain tissue
Positive IHC detected in	mouse brain tissue, human brain tissue, human kidney tissue, human lung cancer tissue, mouse ovary tissue, human tonsillitis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 4 publications below
IF	See 2 publications below
CoIP	See 1 publications below

Product Information

11136-1-AP targets GNAI2 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	GNAI2 fusion protein Ag1597 相同性解析による交差性が予測される生物種
Full Name	guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 2
Calculated molecular weight	41 kDa
Observed molecular weight	35-40 kDa
GenBank accession number	BC012138
Gene symbol	GNAI2
Gene ID (NCBI)	2771
RRID	AB_2232442
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

GNAI2, also named as GNAI2B, belongs to the G-alpha family. G(i/o/t/z) subfamily. Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. The G(i) proteins are involved in hormonal regulation of adenylate cyclase: they inhibit the cyclase in response to beta-adrenergic stimuli. GNAI2 is 93% homolog to GNAI1, 94% to GNAI3, 85% to GNAT3, 82% to GNAT2.

Protocols

Product Specific Protocols
WB protocol for GNAI2 antibody 11136-1-AP	Download protocol
IHC protocol for GNAI2 antibody 11136-1-AP	Download protocol
IF protocol for GNAI2 antibody 11136-1-AP	Download protocol
IP protocol for GNAI2 antibody 11136-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Nat Commun EMX2-GPR156-Gαi reverses hair cell orientation in mechanosensory epithelia. Authors - Katie S Kindt View Article
mouse	IF	Elife Inhibitory G proteins play multiple roles to polarize sensory hair cell morphogenesis Authors - Amandine Jarysta View Article
human	WB	Oncotarget PMID: 24658464 Authors - Jie Li View Article
mouse	IF	bioRxiv Inhibitory G proteins play multiple roles to polarize sensory hair cell morphogenesis Authors - Amandine Jarysta View Article
mouse	WB	Adv Sci (Weinh) Targeting Squalene Epoxidase Confers Metabolic Vulnerability and Overcomes Chemoresistance in HNSCC Authors - Xinyuan Zhao View Article
human	WB,CoIP	Sci Adv RGS12 polarizes the GPSM2-GNAI complex to organize and elongate stereocilia in sensory hair cells Authors - Anil Akturk View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GNAI2 Polyclonal antibody

GNAI2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Validation Data Gallery

WB analysis using 11136-1-AP

WB analysis of fetal human brain using 11136-1-AP

WB analysis of SKOV-3 using 11136-1-AP

IP experiment of mouse brain using 11136-1-AP

IHC staining of mouse brain using 11136-1-AP

IHC staining of mouse brain using 11136-1-AP

IHC staining of human ovary cancer using 11136-1-AP

IHC staining of human brain using 11136-1-AP

IHC staining of human brain using 11136-1-AP

IHC staining of human lung cancer using 11136-1-AP

IHC staining of human tonsillitis using 11136-1-AP

IHC staining of human tonsillitis using 11136-1-AP

IHC staining of human lung cancer using 11136-1-AP

IHC staining of mouse ovary using 11136-1-AP

IHC staining of mouse ovary using 11136-1-AP

IHC staining of human kidney using 11136-1-AP

IHC staining of human kidney using 11136-1-AP

IF Staining of HeLa using 11136-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

EMX2-GPR156-Gαi reverses hair cell orientation in mechanosensory epithelia.

Inhibitory G proteins play multiple roles to polarize sensory hair cell morphogenesis

PMID: 24658464

Inhibitory G proteins play multiple roles to polarize sensory hair cell morphogenesis

Targeting Squalene Epoxidase Confers Metabolic Vulnerability and Overcomes Chemoresistance in HNSCC

RGS12 polarizes the GPSM2-GNAI complex to organize and elongate stereocilia in sensory hair cells