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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GNAI2 Monoclonal antibody, PBS Only

GNAI2 Monoclonal Antibody for WB, IF, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

CloneNo.

3F6H5

Cat no : 67007-1-PBS

Synonyms

GIP, GNAI2, GNAI2B, H_LUCA15.1, H_LUCA16.1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GNAI2 Monoclonal antibody (67007-1-Ig)

    WB analysis using 67007-1-Ig (same clone as 67007-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67007-1-Ig (GNAI2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67007-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of U-937 cells using GNAI2 Monoclonal antibody (67007-1-Ig)

    WB analysis of U-937 using 67007-1-Ig (same clone as 67007-1-PBS)

    U-937 cells were subjected to SDS PAGE followed by western blot with 67007-1-Ig (GNAI2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67007-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using GNAI2 Monoclonal antibody (67007-1-Ig)

    IF Staining of A431 using 67007-1-Ig (same clone as 67007-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using 67007-1-Ig (GNAI2 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67007-1-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67007-1-PBS targets GNAI2 in WB, IF, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen GNAI2 fusion protein Ag28560 相同性解析による交差性が予測される生物種
Full Name guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 2
Calculated molecular weight 41 kDa
Observed molecular weight 37 kDa
GenBank accession numberBC012138
Gene symbol GNAI2
Gene ID (NCBI) 2771
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS only
Storage ConditionsStore at -80°C.

Background Information

GNAI2, also named as GNAI2B, belongs to the G-alpha family. G(i/o/t/z) subfamily. Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. The G(i) proteins are involved in hormonal regulation of adenylate cyclase: they inhibit the cyclase in response to beta-adrenergic stimuli. GNAI2 is 93% homolog to GNAI1, 94% to GNAI3, 85% to GNAT3, 82% to GNAT2.