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GNAI3 Polyclonal antibody

GNAI3 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Cat No. 11641-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Guanine nucleotide-binding protein G(i) subunit alpha-3, G(i) alpha-3, 87U6

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -


ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター(国内)に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。


保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive WB detected inrat kidney tissue, A431 cells, mouse brain tissue, human kidney tissue
Positive IHC detected inhuman kidney tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

11641-1-AP targets GNAI3 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GNAI3 fusion protein Ag2229 相同性解析による交差性が予測される生物種
Full Name guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 3
Calculated molecular weight 40 kDa
Observed molecular weight ~40 kDa
GenBank accession numberBC025285
Gene Symbol GNAI3
Gene ID (NCBI) 2773
RRIDAB_2111489
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP08754
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GNAI3 is a member of the Gi group proteins, which belong to the Gα protein family. GNAI3 is involved in several critical biological processes and regulates many cellular activities, including proliferation, differentiation, apoptosis, and migration. For example, GNAI3 can regulate a class of K+ channels in response to hormone and neurotransmitter signals. In addition to its function as a downstream signal pathway switch for receptors on the plasma membrane, GNAI3 has also been shown to localize to the centrosome and affect cytokinesis. These indicate that GNAI3 has important receptor-independent functions. The molecular weight of GNAI3 is 40 kDa. (PMID: 25444921, 34803495)

Protocols

Product Specific Protocols
WB protocol for GNAI3 antibody 11641-1-APDownload protocol
IHC protocol for GNAI3 antibody 11641-1-APDownload protocol
IF protocol for GNAI3 antibody 11641-1-APDownload protocol
FC protocol for GNAI3 antibody 11641-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Sci Signal

Growth factor-dependent phosphorylation of Gαi shapes canonical signaling by G protein-coupled receptors

Authors - Suchismita Roy
mouseWB

Cell Signal

RGS10 physically and functionally interacts with STIM2 and requires store-operated calcium entry to regulate proinflammatory gene expression in microglia.

Authors - Menbere Wendimu
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