Validation Data Gallery
Tested Applications
| Positive WB detected in | rat kidney tissue, A431 cells, mouse brain tissue, human kidney tissue |
| Positive IHC detected in | human kidney tissue, human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
| Positive FC (Intra) detected in | A431 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
Product Information
11641-1-AP targets GNAI3 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | GNAI3 fusion protein Ag2229 相同性解析による交差性が予測される生物種 |
| Full Name | guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 3 |
| Calculated molecular weight | 40 kDa |
| Observed molecular weight | ~40 kDa |
| GenBank accession number | BC025285 |
| Gene Symbol | GNAI3 |
| Gene ID (NCBI) | 2773 |
| RRID | AB_2111489 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P08754 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
GNAI3 is a member of the Gi group proteins, which belong to the Gα protein family. GNAI3 is involved in several critical biological processes and regulates many cellular activities, including proliferation, differentiation, apoptosis, and migration. For example, GNAI3 can regulate a class of K+ channels in response to hormone and neurotransmitter signals. In addition to its function as a downstream signal pathway switch for receptors on the plasma membrane, GNAI3 has also been shown to localize to the centrosome and affect cytokinesis. These indicate that GNAI3 has important receptor-independent functions. The molecular weight of GNAI3 is 40 kDa. (PMID: 25444921, 34803495)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for GNAI3 antibody 11641-1-AP | Download protocol |
| IHC protocol for GNAI3 antibody 11641-1-AP | Download protocol |
| IF protocol for GNAI3 antibody 11641-1-AP | Download protocol |
| FC protocol for GNAI3 antibody 11641-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Sci Signal Growth factor-dependent phosphorylation of Gαi shapes canonical signaling by G protein-coupled receptors | ||
Cell Signal RGS10 physically and functionally interacts with STIM2 and requires store-operated calcium entry to regulate proinflammatory gene expression in microglia. |