GNAO1 Polyclonal antibody
GNAO1 Polyclonal Antibody for WB, IP, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF, IP, ELISA
Conjugate
Unconjugated
Cat no : 12635-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | mouse brain tissue, Y79 cells, human liver tissue, mouse testis tissue, rat testis tissue, rat brain tissue |
Positive IP detected in | mouse brain tissue |
Positive IHC detected in | human gliomas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:16000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
WB | See 4 publications below |
IHC | See 4 publications below |
IF | See 3 publications below |
Product Information
12635-1-AP targets GNAO1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | GNAO1 fusion protein Ag3330 相同性解析による交差性が予測される生物種 |
Full Name | guanine nucleotide binding protein (G protein), alpha activating activity polypeptide O |
Calculated molecular weight | 302 aa, 35 kDa |
Observed molecular weight | 40 kDa |
GenBank accession number | BC030027 |
Gene symbol | GNAO1 |
Gene ID (NCBI) | 2775 |
RRID | AB_2111635 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
GNAO1 gene encodes the α subunit of heterotrimeric guanine nucleotide-binding protein (Gi protein), a membrane protein widely expressed in the central nervous system involved in signal transduction (PMID: 30642806). GNAO1 belongs to the G-alpha family and G(i/o/t/z) subfamily. GNAO1 is highly expressed in brain and modulate neuronal excitability (PMID: 30838255). GNAO1 has 2 isoforms with the molecular mass of 40 kDa.
Protocols
Product Specific Protocols | |
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WB protocol for GNAO1 antibody 12635-1-AP | Download protocol |
IHC protocol for GNAO1 antibody 12635-1-AP | Download protocol |
IP protocol for GNAO1 antibody 12635-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Blood Identification of functional cooperative mutations of GNAO1 in human acute lymphoblastic leukemia. | ||
Elife Inhibitory G proteins play multiple roles to polarize sensory hair cell morphogenesis | ||
Neurochem Res Efficacy of Hybrid Tetrahydrobenzo[d]thiazole Based Aryl Piperazines D-264 and D-301 at D₂ and D₃ Receptors. | ||
Int J Mol Med Overexpression of GNAO1 correlates with poor prognosis in patients with gastric cancer and plays a role in gastric cancer cell proliferation and apoptosis. | ||
Biosci Rep The down-regulation of GNAO1 and its promoting role in hepatocellular carcinoma. |