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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GNAO1 Monoclonal antibody

GNAO1 Monoclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Mouse, Rat, Rabbit, Pig, Chicken, Human

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

1B5G2

Cat no : 68027-1-Ig

Synonyms

DKFZp686O0962, G ALPHA o, GNAO, GNAO1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GNAO1 Monoclonal antibody (68027-1-Ig)

    WB analysis using 68027-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68027-1-Ig (GNAO1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with Lamin B1 Monoclonal antibody (66095-1-Ig) as loading control.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using GNAO1 Monoclonal antibody (68027-1-Ig)

    IHC staining of human gliomas using 68027-1-Ig

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 68027-1-Ig (GNAO1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human gliomas tissue using GNAO1 Monoclonal antibody (68027-1-Ig)

    IHC staining of human gliomas using 68027-1-Ig

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 68027-1-Ig (GNAO1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of rat cerebellum tissue using GNAO1 Monoclonal antibody (68027-1-Ig)

    IF Staining of rat cerebellum using 68027-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed rat cerebellum tissue using GNAO1 antibody (68027-1-Ig, Clone: 1B5G2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

Positive WB detected inpig brain tissue, rabbit brain tissue, rat brain tissue, mouse brain tissue, chicken brain tissue, rat cerebellum tissue
Positive IHC detected inhuman gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inrat cerebellum tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68027-1-Ig targets GNAO1 in WB, IHC, IF-P, ELISA applications and shows reactivity with Mouse, Rat, Rabbit, Pig, Chicken, Human samples.

Tested Reactivity Mouse, Rat, Rabbit, Pig, Chicken, Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen GNAO1 fusion protein Ag32045 相同性解析による交差性が予測される生物種
Full Name guanine nucleotide binding protein (G protein), alpha activating activity polypeptide O
Calculated molecular weight 302 aa, 35 kDa
Observed molecular weight40 kDa
GenBank accession numberBC030027
Gene symbol GNAO1
Gene ID (NCBI) 2775
RRIDAB_2918770
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GNAO1 gene encodes the α subunit of heterotrimeric guanine nucleotide-binding protein (Gi protein), a membrane protein widely expressed in the central nervous system involved in signal transduction (PMID: 30642806). GNAO1 belongs to the G-alpha family and G(i/o/t/z) subfamily. GNAO1 is highly expressed in brain and modulate neuronal excitability (PMID: 30838255). GNAO1 has 2 isoforms with the molecular mass of 40 kDa.

Protocols

Product Specific Protocols
WB protocol for GNAO1 antibody 68027-1-IgDownload protocol
IHC protocol for GNAO1 antibody 68027-1-IgDownload protocol
IF protocol for GNAO1 antibody 68027-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols