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KD/KO Validated

GNAQ Polyclonal antibody

Name: GNAQ Polyclonal antibody
Brand: Proteintech
SKU: 13927-1-AP

GNAQ Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 13927-1-AP

Synonyms

Guanine nucleotide-binding protein G(q) subunit alpha, Guanine nucleotide-binding protein alpha-q, GAQ, G ALPHA q, CMC1

Validation Data Gallery

Filter:

AllWBIHCIF/ICC

WB analysis using 13927-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 13927-1-AP (GNAQ antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of mouse lung using 13927-1-AP
mouse lung tissue were subjected to SDS PAGE followed by western blot with 13927-1-AP (GNAQ antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse skin using 13927-1-AP
mouse skin tissue were subjected to SDS PAGE followed by western blot with 13927-1-AP (GNAQ antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 13927-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 13927-1-AP (GNAQ antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IHC staining of human testis using 13927-1-AP
Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 13927-1-AP (GNAQ antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human testis using 13927-1-AP
Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 13927-1-AP (GNAQ antibody) at dilution of 1:200 (under 40x lens).
IHC staining of human breast cancer using 13927-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13927-1-AP (GNAQ antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 13927-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13927-1-AP (GNAQ antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HEK-293 using 13927-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using GNAQ antibody (13927-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).
IF Staining of HEK-293 using 13927-1-AP
Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using GNAQ antibody (13927-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Cat No. 13927-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HeLa cells, human brain tissue, mouse lung tissue, mouse skin tissue, Jurkat cells, NIH/3T3 cells, mouse brain tissue, rat brain tissue
Positive IHC detected in	human testis tissue, human breast cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HEK-293 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:6000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 2 publications below
WB	See 8 publications below
IHC	See 1 publications below
IF	See 3 publications below

Product Information

13927-1-AP targets GNAQ in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	GNAQ fusion protein Ag4947 相同性解析による交差性が予測される生物種
Full Name	guanine nucleotide binding protein (G protein), q polypeptide
Calculated molecular weight	42 kDa
Observed molecular weight	38-40 kDa
GenBank accession number	BC057777
Gene symbol	GNAQ
Gene ID (NCBI)	2776
RRID	AB_2111647
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

GNAQ is a proto-oncogene closely related to the α subunit of guanine nucleotide-binding proteins (G proteins) that serve as key intermediates between membrane-bound G-protein-coupled receptors (GPCRs) and GPCR signalling nodes. Aberrant expression and activity of G proteins and GPCRs are frequently associated with tumorigenesis. Recent studies show that oncogenic activating mutations in GNAQ are present in 80% of the melanomas. The calculated molecular weight of GNAQ is 42 kDa. This antibody can detect a band of 38-40 kDa in SDS-PAGE. (PMID: 33993733)

Protocols

Product Specific Protocols
WB protocol for GNAQ antibody 13927-1-AP	Download protocol
IHC protocol for GNAQ antibody 13927-1-AP	Download protocol
IF protocol for GNAQ antibody 13927-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	J Exp Med Uncovering a novel role of PLCβ4 in selectively mediating TCR signaling in CD8+ but not CD4+ T cells. Authors - Miwa Sasai View Article
mouse	WB,IF	Elife Gq activity- and β-arrestin-1 scaffolding-mediated ADGRG2/CFTR coupling are required for male fertility. Authors - Dao-Lai Zhang View Article
mouse	WB	Oncogene Copper ionophore elesclomol selectively targets GNAQ/11-mutant uveal melanoma. Authors - Yongyun Li View Article
human	WB	Proteomics iTRAQ-based proteomic analysis of dioscin on human HCT-116 colon cancer cells. Authors - Hao Chen View Article
human	WB,IHC	Oncol Rep G protein subunit α q regulates gastric cancer growth via the p53/p21 and MEK/ERK pathways. Authors - Yizhuo Wang KD Validated View Article
human	WB,IF	J Biol Chem Enhanced membrane binding of oncogenic G protein αqQ209L confers resistance to inhibitor YM-254890 Authors - Clinita E Randolph View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GNAQ Polyclonal antibody

GNAQ Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Validation Data Gallery

WB analysis using 13927-1-AP

WB analysis of mouse lung using 13927-1-AP

WB analysis of mouse skin using 13927-1-AP

WB analysis of human brain using 13927-1-AP

IHC staining of human testis using 13927-1-AP

IHC staining of human testis using 13927-1-AP

IHC staining of human breast cancer using 13927-1-AP

IHC staining of human breast cancer using 13927-1-AP

IF Staining of HEK-293 using 13927-1-AP

IF Staining of HEK-293 using 13927-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Uncovering a novel role of PLCβ4 in selectively mediating TCR signaling in CD8+ but not CD4+ T cells.

Gq activity- and β-arrestin-1 scaffolding-mediated ADGRG2/CFTR coupling are required for male fertility.

Copper ionophore elesclomol selectively targets GNAQ/11-mutant uveal melanoma.

iTRAQ-based proteomic analysis of dioscin on human HCT-116 colon cancer cells.

G protein subunit α q regulates gastric cancer growth via the p53/p21 and MEK/ERK pathways.

Enhanced membrane binding of oncogenic G protein αqQ209L confers resistance to inhibitor YM-254890