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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GNAS Monoclonal antibody, PBS Only

GNAS Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, pig

Applications

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1A9C10

Cat no : 66253-1-PBS

Synonyms

Neuroendocrine secretory protein 55, NESP55, LHAL tetrapeptide, GPIPIRRH peptide, GNAS1

Validation Data Gallery

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  • Western Blot (WB) analysis of fetal human brain tissue using GNAS Monoclonal antibody (66253-1-Ig)

    WB analysis of fetal human brain using 66253-1-Ig (same clone as 66253-1-PBS)

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66253-1-Ig (NESP55,GNAS Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66253-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig brain tissue using GNAS Monoclonal antibody (66253-1-Ig)

    WB analysis of pig brain using 66253-1-Ig (same clone as 66253-1-PBS)

    pig brain tissue were subjected to SDS PAGE followed by western blot with 66253-1-Ig (NESP55,GNAS Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66253-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using GNAS Monoclonal antibody (66253-1-Ig)

    IHC staining of human colon using 66253-1-Ig (same clone as 66253-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66253-1-Ig (GNAS antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66253-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using GNAS Monoclonal antibody (66253-1-Ig)

    IHC staining of human colon using 66253-1-Ig (same clone as 66253-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66253-1-Ig (GNAS antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66253-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human colon cancer tissue using GNAS Monoclonal antibody (66253-1-Ig)

    IF Staining of human colon cancer using 66253-1-Ig (same clone as 66253-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using GNAS antibody (66253-1-Ig, Clone: 1A9C10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66253-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human colon cancer tissue using GNAS Monoclonal antibody (66253-1-Ig)

    IF Staining of human colon cancer using 66253-1-Ig (same clone as 66253-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using GNAS antibody (66253-1-Ig, Clone: 1A9C10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66253-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human colon cancer tissue using GNAS Monoclonal antibody (66253-1-Ig)

    IF Staining of human colon cancer using 66253-1-Ig (same clone as 66253-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using GNAS antibody (66253-1-Ig, Clone: 1A9C10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66253-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using GNAS Monoclonal antibody (66253-1-Ig)

    IF Staining of MCF-7 using 66253-1-Ig (same clone as 66253-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using GNAS antibody (66253-1-Ig, Clone: 1A9C10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66253-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66253-1-PBS targets GNAS in WB, IHC, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human, pig samples.

Tested Reactivity human, pig
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen GNAS fusion protein Ag20530 相同性解析による交差性が予測される生物種
Full Name GNAS complex locus
Calculated molecular weight 45 kDa
Observed molecular weight 46 kDa
GenBank accession numberBC002722
Gene symbol GNAS
Gene ID (NCBI) 2778
RRIDAB_2881641
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Guanine nucleotide binding protein (G protein), alpha stimulating activity polypeptide 1 (GNAS1) is the ubiquitously expressed heterotrimeric G protein that couples receptors to the effector enzyme adenylyl cyclase and is required for receptor-stimulated intracellular cAMP generation. Mutations of Gs(alpha) residues involved in the GTPase reaction that lead to constitutive activation are present in endocrine tumors, fibrous dysplasia of bone, and McCune-Albright syndrome. The molecular weight of Gs(alpha) protein is about 46 kDa.