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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GNB1L Polyclonal antibody (26484-1-AP)

    WB analysis using 26484-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26484-1-AP (GNB1L antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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GNB1L Polyclonal antibody

GNB1L Polyclonal Antibody for WB, ELISA
Cat No. 26484-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

DGCRK3, FKSG1, GNB1L, GY2, KIAA1645, WDR14, WDVCF

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA549 cells, Jurkat cells, MCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

26484-1-AP targets GNB1L in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GNB1L fusion protein Ag24391 相同性解析による交差性が予測される生物種
Full Name guanine nucleotide binding protein (G protein), beta polypeptide 1-like
Calculated molecular weight 327 aa, 36 kDa
Observed molecular weight36 kDa, 18 kDa
GenBank accession numberBC012060
Gene Symbol GNB1L
Gene ID (NCBI) 54584
RRIDAB_3085876
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BYB4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GNB1L (G-protein subunit beta 1 like), located at 22q11.2, encodes a G-protein L-subunit-like polypeptide which is a member of the WD repeat protein family (PMID: 20538345). In humans, the hemizygous deletion of GNB1L can cause sensorimotor gating defects, which are related to schizophrenia and other serious mental diseases (PMID: 32847500). Moreover, GNB1L is a key regulator of DDR signaling via its role as a co-chaperone specifically regulating PIKK proteins (PMID: 37541219). GNB1L has 2 isoforms 36 kDa and 22 kDa, about 22 kDa which may be produced by Alternative splicing.

Protocols

Product Specific Protocols
WB protocol for GNB1L antibody 26484-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols