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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GNB3 Polyclonal antibody (10081-1-AP)

    WB analysis using 10081-1-AP (same clone as 10081-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 10081-1-AP (GNB3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10081-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using GNB3 Polyclonal antibody (10081-1-AP)

    WB analysis using 10081-1-AP (same clone as 10081-1-PBS)

    rat brain tissue were subjected to SDS PAGE followed by western blot with 10081-1-AP (GNB3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10081-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse embryo tissue using GNB3 Polyclonal antibody (10081-1-AP)

    WB analysis of mouse embryo using 10081-1-AP (same clone as 10081-1-PBS)

    mouse embryo tissue were subjected to SDS PAGE followed by western blot with 10081-1-AP (GNB3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10081-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using GNB3 Polyclonal antibody (10081-1-AP)

    IHC staining of human liver cancer using 10081-1-AP (same clone as 10081-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10081-1-AP (GNB3 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 10081-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using GNB3 Polyclonal antibody (10081-1-AP)

    IHC staining of human liver cancer using 10081-1-AP (same clone as 10081-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10081-1-AP (GNB3 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 10081-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using GNB3 Polyclonal antibody (10081-1-AP)

    IF Staining of HepG2 using 10081-1-AP (same clone as 10081-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using GNB3 antibody (10081-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 10081-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

10081-1-PBS targets GNB3 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GNB3 fusion protein Ag0127 相同性解析による交差性が予測される生物種
Full Name guanine nucleotide binding protein (G protein), beta polypeptide 3
Calculated molecular weight 37 kDa
Observed molecular weight 35-37 kDa
GenBank accession numberBC000115
Gene Symbol GNB3
Gene ID (NCBI) 2784
RRIDAB_2263264
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP16520
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Guanine nucleotide-binding proteins (g proteins) are involved as a modulator or transducer in various transmembrane signaling systems, by integrating signals between receptors and effector proteins. G proteins are composed of an alpha, a beta, and a gamma subunit. This gene encodes a 34 kd beta subunit, being expressed in all tissues. Beta subunits are important regulators of alpha subunits, as well as of certain signal transduction receptors and effectors.