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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GNB3 Recombinant antibody (83452-4-RR)

    WB analysis using 83452-4-RR (same clone as 83452-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83452-4-RR (GNB3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83452-4-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HepG2 cells using GNB3 Recombinant antibody (83452-4-RR)

    FC experiment of HepG2 using 83452-4-RR (same clone as 83452-4-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug GNB3 Recombinant antibody (83452-4-RR, Clone:240281D1) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83452-4-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using GNB3 Recombinant antibody (83452-4-RR)

    FC experiment of HeLa using 83452-4-RR (same clone as 83452-4-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug GNB3 Recombinant antibody (83452-4-RR, Clone:240281D1) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83452-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83452-4-RR

    Affinity and Kinetic Characterization of 83452-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83452-4-RR against Human GNB3 were performed. The affinity constant is below 1 pM.

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GNB3 Recombinant antibody, PBS Only

GNB3 Uni-rAbTM Recombinant Antibody for ELISA, WB
Cat No. 83452-4-PBS
Clone No.240281D1

Host / Isotype

Rabbit / IgG

Reactivity

rat, mouse, human

Applications

ELISA, WB

Synonyms

Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-3, 240281D1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83452-4-PBS targets GNB3 in ELISA, WB applications and shows reactivity with rat, mouse, human samples.

Tested Reactivity rat, mouse, human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen GNB3 fusion protein Ag0127 相同性解析による交差性が予測される生物種
Full Name guanine nucleotide binding protein (G protein), beta polypeptide 3
Calculated molecular weight 37 kDa
GenBank accession numberBC000115
Gene Symbol GNB3
Gene ID (NCBI) 2784
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP16520
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.