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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GNB4 Polyclonal antibody (11978-2-AP)

    WB analysis using 11978-2-AP (same clone as 11978-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 11978-2-AP (GNB4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11978-2-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using GNB4 Polyclonal antibody (11978-2-AP)

    IP experiment of mouse brain using 11978-2-AP (same clone as 11978-2-PBS)

    IP result of anti-GNB4 (IP:11978-2-AP, 4ug; Detection:11978-2-AP 1:800) with mouse brain tissue lysate 4000ug. This data was developed using the same antibody clone with 11978-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using GNB4 Polyclonal antibody (11978-2-AP)

    IF Staining of HepG2 using 11978-2-AP (same clone as 11978-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using GNB4 antibody (11978-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 11978-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using GNB4 Polyclonal antibody (11978-2-AP)

    IF Staining of SH-SY5Y using 11978-2-AP (same clone as 11978-2-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using GNB4 antibody (11978-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 11978-2-PBS in a different storage buffer formulation.

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GNB4 Polyclonal antibody, PBS Only

GNB4 Polyclonal Antibody for WB, IF/ICC, IP, Indirect ELISA
Cat No. 11978-2-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, IP, Indirect ELISA

Synonyms

HG2B, Guanine nucleotide-binding protein subunit beta-4, CMTD1F

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

11978-2-PBS targets GNB4 in WB, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GNB4 fusion protein Ag2604 相同性解析による交差性が予測される生物種
Full Name guanine nucleotide binding protein (G protein), beta polypeptide 4
Calculated molecular weight 340 aa, 38 kDa
Observed molecular weight 38 kDa
GenBank accession numberBC000873
Gene Symbol GNB4
Gene ID (NCBI) 59345
RRIDAB_2109620
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9HAV0
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

G protein subunit beta 4 (GNB4), is one of the heterotrimeric guanine nucleotide-binding proteins (G proteins), which integrate signals between receptors and effector proteins. GNB4 is important regulators of alpha subunits, as well as of certain signal transduction receptors and effectors.