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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using GNB5 Polyclonal antibody (11045-2-AP)

    WB analysis of mouse brain using 11045-2-AP (same clone as 11045-2-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 11045-2-AP (GNB5 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11045-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human brain tissue using GNB5 Polyclonal antibody (11045-2-AP)

    WB analysis of human brain using 11045-2-AP (same clone as 11045-2-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 11045-2-AP (GNB5 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11045-2-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of rat brain tissue using GNB5 Polyclonal antibody (11045-2-AP)

    IP experiment of rat brain using 11045-2-AP (same clone as 11045-2-PBS)

    IP result of anti-GNB5 (IP:11045-2-AP, 4ug; Detection:11045-2-AP 1:500) with rat brain tissue lysate 4000ug. This data was developed using the same antibody clone with 11045-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using GNB5 Polyclonal antibody (11045-2-AP)

    IHC staining of human ovary tumor using 11045-2-AP (same clone as 11045-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 11045-2-AP (GNB5 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11045-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using GNB5 Polyclonal antibody (11045-2-AP)

    IHC staining of human ovary tumor using 11045-2-AP (same clone as 11045-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 11045-2-AP (GNB5 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11045-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using GNB5 Polyclonal antibody (11045-2-AP)

    IHC staining of human ovary tumor using 11045-2-AP (same clone as 11045-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor using 11045-2-AP (GNB5 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 11045-2-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

GNB5 Polyclonal antibody, PBS Only

GNB5 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 11045-2-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

11045-2-PBS targets GNB5 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GNB5 fusion protein Ag1518 相同性解析による交差性が予測される生物種
Full Name guanine nucleotide binding protein (G protein), beta 5
Calculated molecular weight 39 kDa
Observed molecular weight 39-42 kDa
GenBank accession numberBC011671
Gene Symbol GNB5
Gene ID (NCBI) 10681
RRIDAB_2109639
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO14775
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction. G proteins, which integrate signals between receptors and effector proteins, are composed of an alpha, a beta, and a gamma subunit.