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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GNG13 Polyclonal antibody (18223-1-AP)

    WB analysis using 18223-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 18223-1-AP (GNG13 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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GNG13 Polyclonal antibody

GNG13 Polyclonal Antibody for WB, ELISA
Cat No. 18223-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

h2 35, Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-13, G(gamma)13

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse brain tissue, mouse retina tissue, rat brain tissue, rat retina tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

18223-1-AP targets GNG13 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GNG13 fusion protein Ag13007 相同性解析による交差性が予測される生物種
Full Name guanine nucleotide binding protein (G protein), gamma 13
Calculated molecular weight 67 aa, 8 kDa
Observed molecular weight8 kDa
GenBank accession numberBC093760
Gene Symbol GNG13
Gene ID (NCBI) 51764
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9P2W3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GNG13 (Guanine nucleotide-binding protein G (G gamma 13)) is a gene that encodes a gamma subunit of a heterotrimeric G protein. It is expressed in taste, retinal, and neuronal tissues and is crucial for taste transduction. In the main olfactory epithelium (MOE), most olfactory sensory neurons (OSNs) express G proteins consisting of Gαolf and Gβ1γ13. Conditional knockout of the Gng13 gene in mice significantly altered the MOE's gene expression profile and impaired the mutant animals' food - seeking capabilities (PMID: 30157062).

Protocols

Product Specific Protocols
WB protocol for GNG13 antibody 18223-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols