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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GNG7 Polyclonal antibody (31393-1-AP)

    WB analysis using 31393-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31393-1-AP (GNG7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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GNG7 Polyclonal antibody

GNG7 Polyclonal Antibody for WB, ELISA
Cat No. 31393-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

Mouse, Rat

Applications

WB, ELISA

Synonyms

FLJ00058, GNG7, GNGT7

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

31393-1-AP targets GNG7 in WB, ELISA applications and shows reactivity with Mouse, Rat samples.

Tested Reactivity Mouse, Rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GNG7 fusion protein Ag34754 相同性解析による交差性が予測される生物種
Full Name guanine nucleotide binding protein (G protein), gamma 7
Calculated molecular weight 8 kDa
Observed molecular weight10 kDa
GenBank accession numberBC014466
Gene Symbol GNG7
Gene ID (NCBI) 2788
RRIDAB_3669962
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDO60262
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GNG7 belongs to the large guanine nucleotide binding protein γ family and is located on chromosome 19. Related genes of the G protein family are involved in various transmembrane signaling transduction pathways. GNG7 may be associated with transmembrane signaling pathways and involved in cell contact-induced growth arrest, thus repressing uncontrolled cell proliferation in multicellular organisms. GNG7 is a heterotrimeric G protein subunit with ubiquitous expression in various tissues but low expression in cancer. GNG7 overexpression inhibited cell growth and tumorigenicity of esophageal carcinoma cells. Also, GNG7 was confirmed as an essential autophagy-inducing agent and participated in inhibiting tumor progression through mTOR pathway (PMID: 30864685, 35281820, 36159963).

Protocols

Product Specific Protocols
WB protocol for GNG7 antibody 31393-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols