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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse eye tissue using GNGT1 Polyclonal antibody (11884-1-AP)

    WB analysis of mouse eye using 11884-1-AP (same clone as 11884-1-PBS)

    mouse eye tissue were subjected to SDS PAGE followed by western blot with 11884-1-AP (GNGT1 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11884-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using GNGT1 Polyclonal antibody (11884-1-AP)

    IF Staining of HeLa using 11884-1-AP (same clone as 11884-1-PBS)

    Immunofluorescent analysis of HeLa cells using 11884-1-AP (GNGT1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11884-1-PBS in a different storage buffer formulation.

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GNGT1 Polyclonal antibody, PBS Only

GNGT1 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 11884-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

11884-1-PBS targets GNGT1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GNGT1 fusion protein Ag2488 相同性解析による交差性が予測される生物種
Full Name guanine nucleotide binding protein (G protein), gamma transducing activity polypeptide 1
Calculated molecular weight 74 aa, 8 kDa
Observed molecular weight 8 kDa
GenBank accession numberBC029367
Gene Symbol GNGT1
Gene ID (NCBI) 2792
RRIDAB_10858628
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP63211
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

G protein subunit gamma-T1(GNGT1) is encoded by the transducin gamma-subunit gene, which localized on huaman chromosome 7q21.3, participating in various transmembrane signaling systems. Particularly, GNGT1 gene is specific to retinal rod photoreceptors. Present polyclonal anti-GNGT1 antibody (11884-1-AP) is produced by immunizing rabbits with full-length chain of GNGT1 and dectects an 8 kDa band in mouse retinal tissue.