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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GNGT2 Polyclonal antibody

GNGT2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11988-1-AP

Synonyms

GNGT8, GNG9, GNG8, G-gamma-9, G-gamma-8

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GNGT2 Polyclonal antibody (11988-1-AP)

    WB analysis using 11988-1-AP

    Mouse retina tissue were subjected to SDS PAGE followed by western blot with 11988-1-AP (GNGT2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse eye tissue using GNGT2 Polyclonal antibody (11988-1-AP)

    IHC staining of mouse eye using 11988-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 11988-1-AP (GNGT2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse eye tissue using GNGT2 Polyclonal antibody (11988-1-AP)

    IHC staining of mouse eye using 11988-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 11988-1-AP (GNGT2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse eye tissue using GNGT2 Polyclonal antibody (11988-1-AP)

    IHC staining of mouse eye using 11988-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 11988-1-AP (GNGT2 antibody) at dilution of 1:200 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inmouse retina tissue
Positive IHC detected inmouse eye tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11988-1-AP targets GNGT2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GNGT2 fusion protein Ag2618 相同性解析による交差性が予測される生物種
Full Name guanine nucleotide binding protein (G protein), gamma transducing activity polypeptide 2
Calculated molecular weight 69 aa, 8 kDa
Observed molecular weight 8 kDa
GenBank accession numberBC008663
Gene symbol GNGT2
Gene ID (NCBI) 2793
RRIDAB_2877813
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GNGT2, also known as GNG8, GNG9 and GNGT8, belongs to the G protein gamma family. Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction. This antibody has weak cross-reactivity of GNGT1 protein, as the immunogen of GNGT2 shares about 65% homology with GNGT1.

Protocols

Product Specific Protocols
WB protocol for GNGT2 antibody 11988-1-APDownload protocol
IHC protocol for GNGT2 antibody 11988-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols