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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GNL2 Polyclonal antibody (10190-2-AP)

    WB analysis using 10190-2-AP (same clone as 10190-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 10190-2-AP (GNL2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10190-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using GNL2 Polyclonal antibody (10190-2-AP)

    IHC staining of human breast cancer using 10190-2-AP (same clone as 10190-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 10190-2-AP (GNL2 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 10190-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using GNL2 Polyclonal antibody (10190-2-AP)

    IF Staining of A431 using 10190-2-AP (same clone as 10190-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using GNL2 antibody (10190-2-AP) at dilution of 1:2000 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 10190-2-PBS in a different storage buffer formulation.

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GNL2 Polyclonal antibody, PBS Only

GNL2 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 10190-2-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

10190-2-PBS targets GNL2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GNL2 fusion protein Ag0243 相同性解析による交差性が予測される生物種
Full Name guanine nucleotide binding protein-like 2 (nucleolar)
Calculated molecular weight 731 aa, 84 kDa
Observed molecular weight100 kDa
GenBank accession numberBC000107
Gene Symbol GNL2
Gene ID (NCBI) 29889
RRIDAB_2110075
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ13823
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

GNL2 ( G protein nucleolar 2) has a crucial role in the pathogenesis of gliomas by influencing key cellular processes, particularly enhanced protein synthesis, which ultimately contributes to the development of gliomas. In addition, GNL2 was found to affect the overall protein synthesis of ribosomal protein L11 in glioma cells. The calculated MW of GNL2 is 84 kDa, but the observed MW is around 100 kDa, which may be due to glycosylation modification.