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  • Featured Product
  • KD/KO Validated

GNL3 Polyclonal antibody

GNL3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 15060-1-AP

Synonyms

NNP47, Guanine nucleotide-binding protein-like 3, E2-induced gene 3 protein, E2IG3, E2 induced gene 3 protein

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GNL3 Polyclonal antibody (15060-1-AP)

    WB analysis using 15060-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15060-1-AP (Nucleostemin antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using GNL3 Polyclonal antibody (15060-1-AP)

    WB analysis of K-562 using 15060-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 15060-1-AP (Nucleostemin antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using GNL3 Polyclonal antibody (15060-1-AP)

    WB analysis of HeLa using 15060-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 15060-1-AP (Nucleostemin antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using GNL3 Polyclonal antibody (15060-1-AP)

    IP experiment of HeLa using 15060-1-AP

    IP result of anti-GNL3 (IP:15060-1-AP, 4ug; Detection:15060-1-AP 1:600) with HeLa cells lysate 4000ug.

  • Immunohistochemistry (IHC) staining of human oesophagus cancer tissue using GNL3 Polyclonal antibody (15060-1-AP)

    IHC staining of human oesophagus cancer using 15060-1-AP

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 15060-1-AP (GNL3 antibody) at dilution of 1:1600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human oesophagus cancer tissue using GNL3 Polyclonal antibody (15060-1-AP)

    IHC staining of human oesophagus cancer using 15060-1-AP

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 15060-1-AP (GNL3 antibody) at dilution of 1:1600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using GNL3 Polyclonal antibody (15060-1-AP)

    IHC staining of human colon cancer using 15060-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15060-1-AP (GNL3 antibody) at dilution of 1:1600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using GNL3 Polyclonal antibody (15060-1-AP)

    IHC staining of human colon cancer using 15060-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15060-1-AP (GNL3 antibody) at dilution of 1:1600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using GNL3 Polyclonal antibody (15060-1-AP)

    IF Staining of HeLa using 15060-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 15060-1-AP (GNL3 antibody), at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inHEK-293 cells, K-562 cells, HeLa cells, HepG2 cells, Raji cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman oesophagus cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:800-1:3200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15060-1-AP targets GNL3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GNL3 fusion protein Ag7056 相同性解析による交差性が予測される生物種
Full Name guanine nucleotide binding protein-like 3 (nucleolar)
Calculated molecular weight 62 kDa
Observed molecular weight 62 kDa
GenBank accession numberBC001024
Gene symbol GNL3
Gene ID (NCBI) 26354
RRIDAB_2263303
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for GNL3 antibody 15060-1-APDownload protocol
IHC protocol for GNL3 antibody 15060-1-APDownload protocol
IF protocol for GNL3 antibody 15060-1-APDownload protocol
IP protocol for GNL3 antibody 15060-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Nanobiotechnology

Targeted micelles with chemotherapeutics and gene drugs to inhibit the G1/S and G2/M mitotic cycle of prostate cancer.

Authors - Yiran Zhang
View Article
humanWB

Cancer Manag Res

The oncogenic role of GNL3 in the progression and metastasis of osteosarcoma.

Authors - Tianyou Li
  • KD Validated
View Article