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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GNL3 Monoclonal antibody (67169-1-Ig)

    WB analysis using 67169-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67169-1-Ig (GNL3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using GNL3 Monoclonal antibody (67169-1-Ig)

    IF Staining of HeLa using 67169-1-Ig

    Immunofluorescent analysis of 4% PFA fixed HeLa cells using 67169-1-Ig (GNL3 mouse antibody, green) at dilution of 1:200 and CoraLite488-conjugated Goat Anti-Mouse IgG(H+L). F-actin is stained using CL555-phalloidin (red) and DNA is stained by DAPI (blue).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using GNL3 Monoclonal antibody (67169-1-Ig)

    IF Staining of HeLa using 67169-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 67169-1-Ig (GNL3 antibody) at dilution of 1:100 and CoraLite594-Conjugated Goat Anti-Mouse IgG(H+L).

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GNL3 Monoclonal antibody

GNL3 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 67169-1-Ig
Clone No.3B8F10
Publications(1)

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat and More (1)

Applications

WB, IF/ICC, ELISA

Synonyms

C77032, E2 induced gene 3 protein, E2IG3, GNL3, NNP47, Novel nucleolar protein 47, NS, Nucleostemin

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite®594
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, MCF-7 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:20000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67169-1-Ig targets GNL3 in WB, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Cited Reactivityhuman
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen GNL3 fusion protein Ag7056 相同性解析による交差性が予測される生物種
Full Name guanine nucleotide binding protein-like 3 (nucleolar)
Calculated molecular weight 62 kDa
Observed molecular weight 62 kDa
GenBank accession numberBC001024
Gene Symbol GNL3
Gene ID (NCBI) 26354
RRIDAB_2882465
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9BVP2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for GNL3 antibody 67169-1-IgDownload protocol
IF protocol for GNL3 antibody 67169-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

Int J Oral Sci

Integrative single-cell and bulk transcriptomes analyses reveals heterogeneity of serine-glycine-one-carbon metabolism with distinct prognoses and therapeutic vulnerabilities in HNSCC

Authors - Lixuan Wang
View Article