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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GNMT Polyclonal antibody, PBS Only

GNMT Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 18790-1-PBS

Synonyms

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of rat liver tissue using GNMT Polyclonal antibody (18790-1-AP)

    WB analysis of rat liver using 18790-1-AP (same clone as 18790-1-PBS)

    rat liver tissue were subjected to SDS PAGE followed by western blot with 18790-1-AP (GNMT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18790-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using GNMT Polyclonal antibody (18790-1-AP)

    WB analysis using 18790-1-AP (same clone as 18790-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 18790-1-AP (GNMT antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18790-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using GNMT Polyclonal antibody (18790-1-AP)

    WB analysis using 18790-1-AP (same clone as 18790-1-PBS)

    LNCaP cells were subjected to SDS PAGE followed by western blot with 18790-1-AP (GNMT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18790-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using GNMT Polyclonal antibody (18790-1-AP)

    WB analysis using 18790-1-AP (same clone as 18790-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 18790-1-AP (GNMT antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18790-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse liver tissue using GNMT Polyclonal antibody (18790-1-AP)

    IP experiment of mouse liver using 18790-1-AP (same clone as 18790-1-PBS)

    IP result of anti-GNMT (IP:18790-1-AP, 4ug; Detection:18790-1-AP 1:8000) with mouse liver tissue lysate 3040 ug. This data was developed using the same antibody clone with 18790-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse liver tissue using GNMT Polyclonal antibody (18790-1-AP)

    IP experiment of mouse liver using 18790-1-AP (same clone as 18790-1-PBS)

    IP result of anti-GNMT (IP:18790-1-AP, 4ug; Detection:18790-1-AP 1:8000) with mouse liver tissue lysate 1840 ug. This data was developed using the same antibody clone with 18790-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate hyperplasia tissue using GNMT Polyclonal antibody (18790-1-AP)

    IHC staining of human prostate hyperplasia using 18790-1-AP (same clone as 18790-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 18790-1-AP (GNMT antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 18790-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using GNMT Polyclonal antibody (18790-1-AP)

    IHC staining of human liver cancer using 18790-1-AP (same clone as 18790-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18790-1-AP (GNMT antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 18790-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using GNMT Polyclonal antibody (18790-1-AP)

    IHC staining of human liver cancer using 18790-1-AP (same clone as 18790-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18790-1-AP (GNMT antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 18790-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

18790-1-PBS targets GNMT in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GNMT fusion protein Ag4598 相同性解析による交差性が予測される生物種
Full Name glycine N-methyltransferase
Calculated molecular weight 295 aa, 33 kDa
Observed molecular weight 33 kDa
GenBank accession numberBC032627
Gene symbol GNMT
Gene ID (NCBI) 27232
RRIDAB_10597093
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Glycine N-methyltransferase (GNMT, EC 2.1.1.20) was found originally as an enzyme regulating the ratio of SAM to S-adenosyl- homocysteine. GNMT is conservative among different animal species. Glycine-N methyltransferase (GNMT) is a potential tumor suppressor that is commonly inactivated in human hepatoma. GNMT is abundant in liver, but very low in HepG2 cells (PMID: 12566990).