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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GNMT Polyclonal antibody, PBS Only

GNMT Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 18790-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of rat liver tissue using GNMT Polyclonal antibody (18790-1-AP)

    WB analysis of rat liver using 18790-1-AP (same clone as 18790-1-PBS)

    rat liver tissue were subjected to SDS PAGE followed by western blot with 18790-1-AP (GNMT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18790-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using GNMT Polyclonal antibody (18790-1-AP)

    WB analysis using 18790-1-AP (same clone as 18790-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 18790-1-AP (GNMT antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18790-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using GNMT Polyclonal antibody (18790-1-AP)

    WB analysis using 18790-1-AP (same clone as 18790-1-PBS)

    LNCaP cells were subjected to SDS PAGE followed by western blot with 18790-1-AP (GNMT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18790-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using GNMT Polyclonal antibody (18790-1-AP)

    WB analysis using 18790-1-AP (same clone as 18790-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 18790-1-AP (GNMT antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18790-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse liver tissue using GNMT Polyclonal antibody (18790-1-AP)

    IP experiment of mouse liver using 18790-1-AP (same clone as 18790-1-PBS)

    IP result of anti-GNMT (IP:18790-1-AP, 4ug; Detection:18790-1-AP 1:8000) with mouse liver tissue lysate 3040 ug. This data was developed using the same antibody clone with 18790-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse liver tissue using GNMT Polyclonal antibody (18790-1-AP)

    IP experiment of mouse liver using 18790-1-AP (same clone as 18790-1-PBS)

    IP result of anti-GNMT (IP:18790-1-AP, 4ug; Detection:18790-1-AP 1:8000) with mouse liver tissue lysate 1840 ug. This data was developed using the same antibody clone with 18790-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate hyperplasia tissue using GNMT Polyclonal antibody (18790-1-AP)

    IHC staining of human prostate hyperplasia using 18790-1-AP (same clone as 18790-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 18790-1-AP (GNMT antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 18790-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using GNMT Polyclonal antibody (18790-1-AP)

    IHC staining of human liver cancer using 18790-1-AP (same clone as 18790-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18790-1-AP (GNMT antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 18790-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using GNMT Polyclonal antibody (18790-1-AP)

    IHC staining of human liver cancer using 18790-1-AP (same clone as 18790-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18790-1-AP (GNMT antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 18790-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

18790-1-PBS targets GNMT in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GNMT fusion protein Ag4598 相同性解析による交差性が予測される生物種
Full Name glycine N-methyltransferase
Calculated molecular weight 295 aa, 33 kDa
Observed molecular weight 33 kDa
GenBank accession numberBC032627
Gene symbol GNMT
Gene ID (NCBI) 27232
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Glycine N-methyltransferase (GNMT, EC 2.1.1.20) was found originally as an enzyme regulating the ratio of SAM to S-adenosyl- homocysteine. GNMT is conservative among different animal species. Glycine-N methyltransferase (GNMT) is a potential tumor suppressor that is commonly inactivated in human hepatoma. GNMT is abundant in liver, but very low in HepG2 cells (PMID: 12566990).