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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GNRHR Polyclonal antibody (19950-1-AP)

    WB analysis using 19950-1-AP (same clone as 19950-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 19950-1-AP (GNRHR antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19950-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse ovary tissue using GNRHR Polyclonal antibody (19950-1-AP)

    IHC staining of mouse ovary using 19950-1-AP (same clone as 19950-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 19950-1-AP (GNRHR antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19950-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse ovary tissue using GNRHR Polyclonal antibody (19950-1-AP)

    IHC staining of mouse ovary using 19950-1-AP (same clone as 19950-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 19950-1-AP (GNRHR antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19950-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using GNRHR Polyclonal antibody (19950-1-AP)

    IHC staining of human testis using 19950-1-AP (same clone as 19950-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis using 19950-1-AP (GNRHR antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 19950-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using GNRHR Polyclonal antibody (19950-1-AP)

    IHC staining of human testis using 19950-1-AP (same clone as 19950-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis using 19950-1-AP (GNRHR antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 19950-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using GNRHR Polyclonal antibody (19950-1-AP)

    IHC staining of mouse testis using 19950-1-AP (same clone as 19950-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 19950-1-AP (GNRHR Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 19950-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tissue using GNRHR Polyclonal antibody (19950-1-AP)

    IHC staining of human ovary using 19950-1-AP (same clone as 19950-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tissue slide using 19950-1-AP (GNRHR Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 19950-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tissue using GNRHR Polyclonal antibody (19950-1-AP)

    IHC staining of human ovary using 19950-1-AP (same clone as 19950-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tissue slide using 19950-1-AP (GNRHR Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 19950-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using GNRHR Polyclonal antibody (19950-1-AP)

    IHC staining of mouse testis using 19950-1-AP (same clone as 19950-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 19950-1-AP (GNRHR Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 19950-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of MCF-7 cells using GNRHR Polyclonal antibody (19950-1-AP)

    FC experiment of MCF-7 using 19950-1-AP (same clone as 19950-1-PBS)

    1X10^6 MCF-7 cells were intracellularly stained with 0.2 ug Anti-Human GNRHR (19950-1-AP) (red) or 0.2 ug Control Antibody (blue), and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 19950-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

19950-1-PBS targets GNRHR in WB, IHC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name GnRH receptor
Calculated molecular weight 38 kDa
Observed molecular weight60-70 kDa
GenBank accession numberNM_000406
Gene Symbol GNRHR
Gene ID (NCBI) 2798
RRIDAB_10644155
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP30968
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

GNRHR, also named GRHR, belongs to the G-protein coupled receptor 1 family. GNRHR is a receptor for GnRH that mediates the action of GnRH to stimulate the secretion of the gonadotropic hormones luteinizing hormone (LH) and follicle-stimulating hormone (FSH). It mediates its action by association with G-proteins that activate a phosphatidylinositol-calcium second messenger system. Isoform2 of GNRHR may act as an inhibitor of GnRH-R signaling. Defects in GNRHR are a cause of idiopathic hypogonadotropic hypogonadism (IHH). Defects in GNRHR are a cause of fertile eunuch syndrome. The antibody only recognizes the isoform1 of GNRHR. The predicted unmodified molecular weight of the human GNRHR is ~38 kDa, the larger band (50-65 kDa) is likely to represent a glycosylated form of GNRHR.