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Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using GOLGA2/GM130 Monoclonal antibody (66662-1-Ig)

    WB analysis of HEK-293 using 66662-1-Ig (same clone as 66662-1-PBS)

    WB result of GOLGA2/GM130 antibody (66662-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GOLGA2/GM130 transfected HEK-293 cells. This data was developed using the same antibody clone with 66662-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using GOLGA2/GM130 Monoclonal antibody (66662-1-Ig)

    WB analysis of HeLa using 66662-1-Ig (same clone as 66662-1-PBS)

    WB result of GOLGA2/GM130 antibody (66662-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GOLGA2/GM130 transfected HeLa cells. This data was developed using the same antibody clone with 66662-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using GOLGA2/GM130 Monoclonal antibody (66662-1-Ig)

    WB analysis using 66662-1-Ig (same clone as 66662-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66662-1-Ig (GOLGA2/GM130 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66662-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using GOLGA2/GM130 Monoclonal antibody (66662-1-Ig)

    WB analysis using 66662-1-Ig (same clone as 66662-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66662-1-Ig (GM130;GOLGA2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66662-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using GOLGA2/GM130 Monoclonal antibody (66662-1-Ig)

    IF Staining of HeLa using 66662-1-Ig (same clone as 66662-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GOLGA2/GM130 antibody (66662-1-Ig, Clone: 2A4F11 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66662-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using GOLGA2/GM130 Monoclonal antibody (66662-1-Ig)

    IF Staining of HeLa using 66662-1-Ig (same clone as 66662-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GOLGA2/GM130 antibody (66662-1-Ig, Clone: 2A4F11 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66662-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP51313-1

    Cytometric bead array standard curve of MP51313-1

    Cytometric bead array standard curve of MP51313-1, GM130;GOLGA2 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66662-1-PBS. Detection antibody: 66662-3-PBS. Standard:Ag1848. Range: 0.781-100 ng/mL.

  • Cytometric bead array standard curve of MP51313-2

    Cytometric bead array standard curve of MP51313-2

    Cytometric bead array standard curve of MP51313-2, GM130;GOLGA2 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66662-1-PBS. Detection antibody: 66662-3-PBS. Standard:Ag1848. Range: 0.781-100 ng/mL.

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  • Featured Product
  • KD/KO Validated

GOLGA2/GM130 Monoclonal antibody, PBS Only (Capture)

GOLGA2/GM130 Monoclonal Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA
Cat No. 66662-1-PBS
Clone No.2A4F11

Host / Isotype

Mouse / IgG1

Reactivity

human, rat

Applications

WB, IF/ICC, Cytometric bead array, Indirect ELISA

Synonyms

GM130, GOLGA2, GM130;GOLGA2, GM130 autoantigen, 2A4F11

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66662-1-PBS targets GOLGA2/GM130 as part of a matched antibody pair:

MP51313-1: 66662-1-PBS capture and 66662-3-PBS detection (validated in Cytometric bead array)

MP51313-2: 66662-1-PBS capture and 66662-3-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen GOLGA2/GM130 fusion protein Ag1848 相同性解析による交差性が予測される生物種
Full Name golgi autoantigen, golgin subfamily a, 2
Calculated molecular weight 111 kDa
Observed molecular weight 130 kDa
GenBank accession numberBC014188
Gene Symbol GM130
Gene ID (NCBI) 2801
RRIDAB_2882017
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ08379
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

GOLGA2, also known as GM130, is a 130 kDa cis-Golgi matrix protein which is one component of the detergent and salt resistant Golgi matrix. It is a peripheral membrane protein highly bound to Golgi membrane and localized mainly at the cytoplasmic face of cis-Golgi membrane. Together with its interacting partner proteins, including p115, giantin, GRASP65, and Rab GTPase, GOLGA2/GM130 is involved in the regulation of ER-to-Golgi transport and also in the maintenance of the Golgi structure. Emerging evidence suggest that the GOLGA2/GM130 has potential roles in the control of glycosylation, cell cycle progression, and higher order cell functions such as cell polarization and directed cell migration. (PMID: 20197635)