GOLGA3 Polyclonal antibody

Name: GOLGA3 Polyclonal antibody
Brand: Proteintech
SKU: 21193-1-AP
Rating: 5.00 (1 reviews)

GOLGA3 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

Reviews (1)

Cat no : 21193-1-AP

Synonyms

MEA2, MEA 2, Golgin-160, Golgin 160, Golgi complex-associated protein of 170 kDa

Validation Data Gallery

Filter:

AllWBIF/ICC

WB analysis of L02 using 21193-1-AP
L02 cells were subjected to SDS PAGE followed by western blot with 21193-1-AP (GOLGA3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 21193-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 21193-1-AP (GOLGA3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of human testis using 21193-1-AP
human testis tissue were subjected to SDS PAGE followed by western blot with 21193-1-AP (GOLGA3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 21193-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 21193-1-AP (GOLGA3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human heart using 21193-1-AP
human heart tissue were subjected to SDS PAGE followed by western blot with 21193-1-AP (GOLGA3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of PC-3 using 21193-1-AP
PC-3 cells were subjected to SDS PAGE followed by western blot with 21193-1-AP (GOLGA3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
IF Staining of HepG2 using 21193-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using GOLGA3 antibody (21193-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Cat No. 21193-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	L02 cells, HEK-293 cells, HeLa cells, human heart tissue, human testis tissue, PC-3 cells
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:6000
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 9 publications below
IF	See 2 publications below

Product Information

21193-1-AP targets GOLGA3 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	GOLGA3 fusion protein Ag15531 相同性解析による交差性が予測される生物種
Full Name	golgi autoantigen, golgin subfamily a, 3
Calculated molecular weight	1498 aa, 167 kDa
Observed molecular weight	167 kDa
GenBank accession number	BC142658
Gene symbol	GOLGA3
Gene ID (NCBI)	2802
RRID	AB_10733640
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for GOLGA3 antibody 21193-1-AP	Download protocol
IF protocol for GOLGA3 antibody 21193-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Proc Natl Acad Sci U S A Aβ-induced Golgi fragmentation in Alzheimer's disease enhances Aβ production. Authors - Gunjan Joshi View Article
human	WB	PLoS Biol A conserved ubiquitin- and ESCRT-dependent pathway internalizes human lysosomal membrane proteins for degradation. Authors - Weichao Zhang View Article
human	WB	J Biol Chem GRASP55 regulates the unconventional secretion and aggregation of mutant huntingtin. Authors - Erpan Ahat View Article
human	WB	Mol Biol Cell Hydrogen peroxide induces Arl1 degradation and impairs Golgi-mediated trafficking. Authors - Stephen C Ireland View Article
human,mouse	WB,IF	PeerJ Mutation of S461, in the GOLGA3 phosphorylation site, does not affect mouse spermatogenesis Authors - Changtong Xu View Article
	WB	Methods Mol Biol Common Markers and Small Molecule Inhibitors in Golgi Studies Authors - Sarah Bui View Article

Reviews

Add a review

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

Boyan (Verified Customer) (04-02-2023)

Very good for WB.

Applications: Western Blot

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GOLGA3 Polyclonal antibody

GOLGA3 Polyclonal Antibody for WB, IF/ICC, ELISA

Validation Data Gallery

WB analysis of L02 using 21193-1-AP

WB analysis of HeLa using 21193-1-AP

WB analysis of human testis using 21193-1-AP

WB analysis of HEK-293 using 21193-1-AP

WB analysis of human heart using 21193-1-AP

WB analysis of PC-3 using 21193-1-AP

IF Staining of HepG2 using 21193-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Aβ-induced Golgi fragmentation in Alzheimer's disease enhances Aβ production.

A conserved ubiquitin- and ESCRT-dependent pathway internalizes human lysosomal membrane proteins for degradation.

GRASP55 regulates the unconventional secretion and aggregation of mutant huntingtin.

Hydrogen peroxide induces Arl1 degradation and impairs Golgi-mediated trafficking.

Mutation of S461, in the GOLGA3 phosphorylation site, does not affect mouse spermatogenesis

Common Markers and Small Molecule Inhibitors in Golgi Studies

Reviews