Validation Data Gallery
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Tested Applications
| Positive WB detected in | HEK-293 cells, human testis tissue, L02 cells, PC-3 cells, HeLa cells |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 10 publications below |
| IF | See 2 publications below |
Product Information
21193-1-AP targets GOLGA3 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | GOLGA3 fusion protein Ag15531 相同性解析による交差性が予測される生物種 |
| Full Name | golgi autoantigen, golgin subfamily a, 3 |
| Calculated molecular weight | 1498 aa, 167 kDa |
| Observed molecular weight | 167 kDa |
| GenBank accession number | BC142658 |
| Gene Symbol | GOLGA3 |
| Gene ID (NCBI) | 2802 |
| RRID | AB_10733640 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q08378 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for GOLGA3 antibody 21193-1-AP | Download protocol |
| IF protocol for GOLGA3 antibody 21193-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Proc Natl Acad Sci U S A Aβ-induced Golgi fragmentation in Alzheimer's disease enhances Aβ production. | ||
PLoS Biol A conserved ubiquitin- and ESCRT-dependent pathway internalizes human lysosomal membrane proteins for degradation. | ||
J Biol Chem GRASP55 regulates the unconventional secretion and aggregation of mutant huntingtin. | ||
Mol Biol Cell Hydrogen peroxide induces Arl1 degradation and impairs Golgi-mediated trafficking. | ||
PeerJ Mutation of S461, in the GOLGA3 phosphorylation site, does not affect mouse spermatogenesis | ||