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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GOLGA5 Recombinant antibody (83839-2-RR)

    WB analysis using 83839-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83839-2-RR (GOLGA5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using GOLGA5 Recombinant antibody (83839-2-RR)

    IHC staining of human ovary cancer using 83839-2-RR

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83839-2-RR (GOLGA5 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 83839-2-RR

    Affinity and Kinetic Characterization of 83839-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83839-2-RR against Human GOLGA5 were performed. The affinity constant is 5.79 nM.

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GOLGA5 Recombinant antibody

GOLGA5 Recombinant Antibody for WB, IHC, ELISA
Cat No. 83839-2-RR
Clone No.240893F6

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

GOLIM5, Golgin-84, Golgin subfamily A member 5, Golgin 84, Cell proliferation-inducing gene 31 protein

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHepG2 cells, L02 cells, HeLa cells, Jurkat cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83839-2-RR targets GOLGA5 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen GOLGA5 fusion protein Ag35031 相同性解析による交差性が予測される生物種
Full Name golgi autoantigen, golgin subfamily a, 5
Calculated molecular weight 83 kDa
Observed molecular weight 80-95 kDa
GenBank accession numberBC023021
Gene Symbol GOLGA5
Gene ID (NCBI) 9950
RRIDAB_3671422
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ8TBA6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GOLGA5 (Golgin subfamily A member 5) maintains Golgi structure, and stimulates the formation of Golgi stacks and ribbons. Also, it is involved in intra-Golgi retrograde transport (PMID: 12538640; 15718469).

Protocols

Product Specific Protocols
WB protocol for GOLGA5 antibody 83839-2-RRDownload protocol
IHC protocol for GOLGA5 antibody 83839-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols