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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GOLPH3 Monoclonal antibody (67777-1-Ig)

    WB analysis using 67777-1-Ig (same clone as 67777-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67777-1-Ig (GOLPH3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67777-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using GOLPH3 Monoclonal antibody (67777-1-Ig)

    WB analysis of HeLa using 67777-1-Ig (same clone as 67777-1-PBS)

    WB result of GOLPH3 antibody (67777-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GOLPH3 transfected HeLa cells. This data was developed using the same antibody clone with 67777-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using GOLPH3 Monoclonal antibody (67777-1-Ig)

    IHC staining of human colon cancer using 67777-1-Ig (same clone as 67777-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67777-1-Ig (GOLPH3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67777-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using GOLPH3 Monoclonal antibody (67777-1-Ig)

    IHC staining of human colon cancer using 67777-1-Ig (same clone as 67777-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67777-1-Ig (GOLPH3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67777-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using GOLPH3 Monoclonal antibody (67777-1-Ig)

    IHC staining of human liver cancer using 67777-1-Ig (same clone as 67777-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67777-1-Ig (GOLPH3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67777-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using GOLPH3 Monoclonal antibody (67777-1-Ig)

    IHC staining of human liver cancer using 67777-1-Ig (same clone as 67777-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67777-1-Ig (GOLPH3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67777-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using GOLPH3 Monoclonal antibody (67777-1-Ig)

    IHC staining of human colon cancer using 67777-1-Ig (same clone as 67777-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67777-1-Ig (GOLPH3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67777-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using GOLPH3 Monoclonal antibody (67777-1-Ig)

    IHC staining of human colon cancer using 67777-1-Ig (same clone as 67777-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67777-1-Ig (GOLPH3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67777-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using GOLPH3 Monoclonal antibody (67777-1-Ig)

    IF Staining of HeLa using 67777-1-Ig (same clone as 67777-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GOLPH3 antibody (67777-1-Ig, Clone: 3H2A5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67777-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

GOLPH3 Monoclonal antibody, PBS Only

GOLPH3 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 67777-1-PBS
Clone No.3H2A5

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

MIDAS, GPP34, GOPP1, GOLPH 3, Golgi phosphoprotein 3

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67777-1-PBS targets GOLPH3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen GOLPH3 fusion protein Ag5443 相同性解析による交差性が予測される生物種
Full Name golgi phosphoprotein 3 (coat-protein)
Calculated molecular weight 298 aa, 34 kDa
Observed molecular weight34 kDa
GenBank accession numberBC033725
Gene Symbol GOLPH3
Gene ID (NCBI) 64083
RRIDAB_2918542
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9H4A6
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

GOLPH3 (also called GPP34, GMx33, MIDAS, or yeast Vps74p) is a 34-kDa Golgi-associated protein conserved from yeast to human. GOLPH3 binds to PtdIns(4)P-rich trans-Golgi membranes and MYO18A conveying a tensile force required for efficient tubule and vesicle formation (PMID: 19837035). GOLPH3 has been recently demonstrated as a novel oncoprotein amplified in various types of human malignancies, including melanoma, breast, non-small cell lung cancer, gliomas and connective tissue tumors (PMID:19553991; 23006319; 21499727; 22745132). Enhanced activation of mTOR signaling represents a molecular basis for the oncogenic activity of GOLPH3 (PMID: 19553991).