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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Syntabulin Polyclonal antibody

Syntabulin Polyclonal Antibody for WB, IF/ICC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 16972-1-AP

Synonyms

Syntaxin-1-binding protein, SYBU, KIAA1472, GOLSYN, Golgi-localized syntaphilin-related protein

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using Syntabulin Polyclonal antibody (16972-1-AP)

    WB analysis of HeLa using 16972-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 16972-1-AP (Syntabulin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using Syntabulin Polyclonal antibody (16972-1-AP)

    WB analysis of MCF-7 using 16972-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 16972-1-AP (Syntabulin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of rat brain tissue using Syntabulin Polyclonal antibody (16972-1-AP)

    IF Staining of rat brain using 16972-1-AP

    Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using 16972-1-AP (Syntabulin antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of rat brain tissue using Syntabulin Polyclonal antibody (16972-1-AP)

    IF Staining of rat brain using 16972-1-AP

    Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using 16972-1-AP (Syntabulin antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Syntabulin Polyclonal antibody (16972-1-AP)

    IF Staining of HeLa using 16972-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 16972-1-AP (Syntabulin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inHeLa cells, MCF-7 cells
Positive IF-P detected inrat brain tissue
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16972-1-AP targets Syntabulin in WB, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Syntabulin fusion protein Ag9344 相同性解析による交差性が予測される生物種
Full Name Golgi-localized protein
Calculated molecular weight 662 aa, 72 kDa
Observed molecular weight 90 kDa
GenBank accession numberBC012082
Gene symbol GOLSYN
Gene ID (NCBI) 55638
RRIDAB_1939519
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for Syntabulin antibody 16972-1-APDownload protocol
IF protocol for Syntabulin antibody 16972-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Hum Mol Genet

Loss of FEZ1, a gene deleted in Jacobsen syndrome, causes locomotion defects and early mortality by impairing motor neuron development.

Authors - Saravanan Gunaseelan
View Article
mouse,humanWB,IF

Cell Death Discov

Syntabulin regulates neuronal excitation/inhibition balance and epileptic seizures by transporting syntaxin 1B

Authors - Pingyang Ke
View Article
mouseWB

Neurobiol Dis

Amyloid beta-mediated KIF5A deficiency disrupts anterograde axonal mitochondrial movement.

Authors - Qi Wang
View Article