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GOPC Polyclonal antibody

GOPC Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 12163-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

PIST, GOPC1, Golgi-associated PDZ and coiled-coil motif-containing protein, FIG, CFTR-associated ligand

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -


ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター(国内)に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。


保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive WB detected inHepG2 cells, PC-3 cells, mouse colon
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293T cells, HEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12163-1-AP targets GOPC in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GOPC fusion protein Ag2804 相同性解析による交差性が予測される生物種
Full Name golgi associated PDZ and coiled-coil motif containing
Calculated molecular weight 454 aa, 50 kDa
Observed molecular weight 50-55 kDa
GenBank accession numberBC009553
Gene Symbol GOPC
Gene ID (NCBI) 57120
RRIDAB_2113182
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9HD26
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GOPC (FIG/PIST/CAL) is a PDZ-domain scaffolding protein that regulates the trafficking of many integral membrane proteins, including cell surface receptors, ion channels and adhesion molecules. GOPC localizes to the trans-Golgi network (TGN) but not to the cis- or trans-Golgi cisternae, thus anti-GOPC can be used to label the TGN structure.

Protocols

Product Specific Protocols
WB protocol for GOPC antibody 12163-1-APDownload protocol
IHC protocol for GOPC antibody 12163-1-APDownload protocol
IF protocol for GOPC antibody 12163-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Cell Mol Life Sci

Golgi pH elevation due to loss of V-ATPase subunit V0a2 function correlates with tissue-specific glycosylation changes and globozoospermia

Authors - Johannes Kopp
human,mouseWB

Environ Int

Repression of autophagy leads to acrosome biogenesis disruption caused by a sub-chronic oral administration of polystyrene nanoparticles.

Authors - Lixiao Zhou
mouseWB

J Cell Mol Med

CCDC157 is essential for sperm differentiation and shows oligoasthenoteratozoospermia-related mutations in men

Authors - Huimei Zheng
mouseIF

Elife

TMC7 deficiency causes acrosome biogenesis defects and male infertility in mice

Authors - Jing Wang
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