Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis using 10598-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10598-1-AP (GORASP2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 10598-1-AP
WB result of GORASP2 antibody (10598-1-AP; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GORASP2 transfected HeLa cells.
WB analysis using 10598-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10598-1-AP (GORASP2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of rat testis using 10598-1-AP
rat testis tissue were subjected to SDS PAGE followed by western blot with 10598-1-AP (GORASP2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 10598-1-AP
IP result of anti-GORASP2 (IP:10598-1-AP, 3ug; Detection:10598-1-AP 1:700) with HeLa cells lysate 1320ug.
IHC staining of human colon cancer using 10598-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer using 10598-1-AP (GORASP2 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human colon cancer using 10598-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10598-1-AP (GORASP2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HepG2 using 10598-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using GORASP2 antibody (10598-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL555-Phalloidin (orange).
IF Staining of HepG2 using 10598-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using GORASP2 antibody (10598-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).
IF Staining of HepG2 using 10598-1-AP
Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 10598-1-AP (GORASP2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Featured Product
KD/KO Validated

GORASP2 Polyclonal antibody

GORASP2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 10598-1-AP

Publications(71)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

GRS2, GRASP55, GOLPH6, Golgi reassembly-stacking protein of 55 kDa, Golgi reassembly-stacking protein 2

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488 CoraLite®594

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (71)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	COLO 320 cells, rat testis tissue, HeLa cells, Raji cells
Positive IP detected in	HeLa cells
Positive IHC detected in	human colon cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:3000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 26 publications below
WB	See 51 publications below
IHC	See 2 publications below
IF	See 35 publications below

Product Information

10598-1-AP targets GORASP2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, monkey
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	GORASP2 fusion protein Ag0950 相同性解析による交差性が予測される生物種
Full Name	golgi reassembly stacking protein 2, 55kDa
Calculated molecular weight	55 kDa
Observed molecular weight	59 kDa
GenBank accession number	BC007770
Gene Symbol	GORASP2
Gene ID (NCBI)	26003
RRID	AB_2113473
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9H8Y8
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

GORASP2 (Golgi reassembly-stacking protein 2, or GRS2) has multiple phosphorylation sites and can be phosphorylated by MKK/ERK pathway during mitoisis. GORASP2 plays a role in the assembly and membrane stacking of the Golgi cisternae, and in the process by which Golgi stacks reform after mitotic breakdown.GORASP2 is supposed to regulate the intracellular transport and presentation of a defined set of transmembrane proteins, such as transmembrane TGFA.

Protocols

Product Specific Protocols
WB protocol for GORASP2 antibody 10598-1-AP	Download protocol
IHC protocol for GORASP2 antibody 10598-1-AP	Download protocol
IF protocol for GORASP2 antibody 10598-1-AP	Download protocol
IP protocol for GORASP2 antibody 10598-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	IHC	Nature Cell stress in cortical organoids impairs molecular subtype specification. Authors - Aparna Bhaduri View Article
human	WB,IF	Mol Cell An mTORC1-GRASP55 signaling axis controls unconventional secretion to reshape the extracellular proteome upon stress. Authors - Julian Nüchel View Article
human	WB	Dev Cell A two-tier Golgi-based control of organelle size underpins the functional plasticity of endothelial cells. Authors - Francesco Ferraro KD Validated View Article
		Nat Commun Land-locked mammalian Golgi reveals cargo transport between stable cisternae. Authors - Myun Hwa Dunlop View Article
mouse	IF	J Clin Invest Anakinra restores cellular proteostasis by coupling mitochondrial redox balance to autophagy Authors - Frank L. van de Veerdonk KD Validated View Article
human	WB,IF	J Cell Biol GRASP55 and GRASP65 play complementary and essential roles in Golgi cisternal stacking. Authors - Xiang Yi Y KD Validated View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 10598-1-AP

WB analysis of HeLa using 10598-1-AP

WB analysis using 10598-1-AP

WB analysis of rat testis using 10598-1-AP

IP experiment of HeLa using 10598-1-AP

IHC staining of human colon cancer using 10598-1-AP

IHC staining of human colon cancer using 10598-1-AP

IF Staining of HepG2 using 10598-1-AP

IF Staining of HepG2 using 10598-1-AP

IF Staining of HepG2 using 10598-1-AP

GORASP2 Polyclonal antibody

GORASP2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Cell stress in cortical organoids impairs molecular subtype specification.

An mTORC1-GRASP55 signaling axis controls unconventional secretion to reshape the extracellular proteome upon stress.

A two-tier Golgi-based control of organelle size underpins the functional plasticity of endothelial cells.

Land-locked mammalian Golgi reveals cargo transport between stable cisternae.

Anakinra restores cellular proteostasis by coupling mitochondrial redox balance to autophagy

GRASP55 and GRASP65 play complementary and essential roles in Golgi cisternal stacking.