☆ 3/31(月)まで!二次抗体 & FlexAbleが30%オフ! >> 免疫アッセイサポートキャンペーン

  • Featured Product
  • KD/KO Validated

GORASP2 Polyclonal antibody

GORASP2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 10598-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

GRS2, GRASP55, GOLPH6, Golgi reassembly-stacking protein of 55 kDa, Golgi reassembly-stacking protein 2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Size/Concentration: 

-/ -


ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。


国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター(国内)に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。


保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive WB detected inCOLO 320 cells, rat testis tissue, HeLa cells, Raji cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10598-1-AP targets GORASP2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, monkey
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GORASP2 fusion protein Ag0950 相同性解析による交差性が予測される生物種
Full Name golgi reassembly stacking protein 2, 55kDa
Calculated molecular weight 55 kDa
Observed molecular weight 59 kDa
GenBank accession numberBC007770
Gene Symbol GORASP2
Gene ID (NCBI) 26003
RRIDAB_2113473
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H8Y8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GORASP2 (Golgi reassembly-stacking protein 2, or GRS2) has multiple phosphorylation sites and can be phosphorylated by MKK/ERK pathway during mitoisis. GORASP2 plays a role in the assembly and membrane stacking of the Golgi cisternae, and in the process by which Golgi stacks reform after mitotic breakdown.GORASP2 is supposed to regulate the intracellular transport and presentation of a defined set of transmembrane proteins, such as transmembrane TGFA.

Protocols

Product Specific Protocols
WB protocol for GORASP2 antibody 10598-1-APDownload protocol
IHC protocol for GORASP2 antibody 10598-1-APDownload protocol
IF protocol for GORASP2 antibody 10598-1-APDownload protocol
IP protocol for GORASP2 antibody 10598-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Nature

Cell stress in cortical organoids impairs molecular subtype specification.

Authors - Aparna Bhaduri
humanWB,IF

Mol Cell

An mTORC1-GRASP55 signaling axis controls unconventional secretion to reshape the extracellular proteome upon stress.

Authors - Julian Nüchel
humanWB

Dev Cell

A two-tier Golgi-based control of organelle size underpins the functional plasticity of endothelial cells.

Authors - Francesco Ferraro
  • KD Validated

Nat Commun

Land-locked mammalian Golgi reveals cargo transport between stable cisternae.

Authors - Myun Hwa Dunlop
mouseIF

J Clin Invest

Anakinra restores cellular proteostasis by coupling mitochondrial redox balance to autophagy

Authors - Frank L. van de Veerdonk
  • KD Validated
humanWB,IF

J Cell Biol

GRASP55 and GRASP65 play complementary and essential roles in Golgi cisternal stacking.

Authors - Xiang Yi Y
  • KD Validated
Loading...