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- KD/KO Validated
GORASP2 Polyclonal antibody
GORASP2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
WB, IHC, IF/ICC, IP, ELISA
Conjugate
Unconjugated
Cat no : 10598-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | COLO 320 cells, rat testis tissue, HeLa cells, Raji cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 26 publications below |
| WB | See 49 publications below |
| IHC | See 2 publications below |
| IF | See 34 publications below |
Product Information
10598-1-AP targets GORASP2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, monkey |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | GORASP2 fusion protein Ag0950 相同性解析による交差性が予測される生物種 |
| Full Name | golgi reassembly stacking protein 2, 55kDa |
| Calculated molecular weight | 55 kDa |
| Observed molecular weight | 59 kDa |
| GenBank accession number | BC007770 |
| Gene symbol | GORASP2 |
| Gene ID (NCBI) | 26003 |
| RRID | AB_2113473 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
GORASP2 (Golgi reassembly-stacking protein 2, or GRS2) has multiple phosphorylation sites and can be phosphorylated by MKK/ERK pathway during mitoisis. GORASP2 plays a role in the assembly and membrane stacking of the Golgi cisternae, and in the process by which Golgi stacks reform after mitotic breakdown.GORASP2 is supposed to regulate the intracellular transport and presentation of a defined set of transmembrane proteins, such as transmembrane TGFA.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for GORASP2 antibody 10598-1-AP | Download protocol |
| IHC protocol for GORASP2 antibody 10598-1-AP | Download protocol |
| IF protocol for GORASP2 antibody 10598-1-AP | Download protocol |
| IP protocol for GORASP2 antibody 10598-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Mol Cell An mTORC1-GRASP55 signaling axis controls unconventional secretion to reshape the extracellular proteome upon stress. | ||
Dev Cell A two-tier Golgi-based control of organelle size underpins the functional plasticity of endothelial cells.
| ||
J Clin Invest Anakinra restores cellular proteostasis by coupling mitochondrial redox balance to autophagy
| ||
J Cell Biol GRASP55 and GRASP65 play complementary and essential roles in Golgi cisternal stacking.
|
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Cassan (Verified Customer) (06-16-2022) | Verified good working antibody
 |
FH Hai (Verified Customer) (07-09-2021) | I used 1:400 dilution in 2%BSA/PBS and got strong signals. The cells in the image I uploaded was stained with mouse-Tubulin- AF633 and rabbit-GORASP2- AF488 and DAPI.
 |
FH Ana (Verified Customer) (03-14-2021) | GORASP, golgi labeling in human primary fibroblasts. PFA 4% fixed cells. 1:100 primary ab in 5% GS PBST (0.1% triton) O/N 4ºC incubation. 1:500 secondary ab Alexafluor 488 1h RT.
 |
FH Uxoa (Verified Customer) (02-19-2020) | GORASP2 (alexa fluor 488, green), Vimentin (alexa fluor 647, white), DAPI (blue). Primary fibroblasts fixed with 4% PFA 15min. 1:100 GORASP2 ab dilution, in PBST (0.1% triton) + 10% DS O/N 4ºC.
 |
