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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GORASP2 Monoclonal antibody, PBS Only

GORASP2 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Rat, Pig

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1C9A3

Cat no : 66627-1-PBS

Synonyms

Golgi phosphoprotein 6, GOLPH6, GORASP2, GRASP55, GRS2, p59

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GORASP2 Monoclonal antibody (66627-1-Ig)

    WB analysis using 66627-1-Ig (same clone as 66627-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66627-1-Ig (GORASP2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66627-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig brain tissue using GORASP2 Monoclonal antibody (66627-1-Ig)

    WB analysis of pig brain using 66627-1-Ig (same clone as 66627-1-PBS)

    pig brain tissue were subjected to SDS PAGE followed by western blot with 66627-1-Ig (GORASP2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66627-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat brain tissue using GORASP2 Monoclonal antibody (66627-1-Ig)

    WB analysis of rat brain using 66627-1-Ig (same clone as 66627-1-PBS)

    rat brain tissue were subjected to SDS PAGE followed by western blot with 66627-1-Ig (GORASP2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66627-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using GORASP2 Monoclonal antibody (66627-1-Ig)

    IHC staining of human colon cancer using 66627-1-Ig (same clone as 66627-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66627-1-Ig (GORASP2 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66627-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using GORASP2 Monoclonal antibody (66627-1-Ig)

    IHC staining of human colon cancer using 66627-1-Ig (same clone as 66627-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66627-1-Ig (GORASP2 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66627-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using GORASP2 Monoclonal antibody (66627-1-Ig)

    IF Staining of HepG2 using 66627-1-Ig (same clone as 66627-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using GORASP2 antibody (66627-1-Ig, Clone: 1C9A3 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66627-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66627-1-PBS targets GORASP2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, Rat, Pig samples.

Tested Reactivity Human, Rat, Pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen GORASP2 fusion protein Ag17607 相同性解析による交差性が予測される生物種
Full Name golgi reassembly stacking protein 2, 55kDa
Calculated molecular weight 55 kDa
Observed molecular weight 59 kDa
GenBank accession numberBC007770
Gene symbol GORASP2
Gene ID (NCBI) 26003
RRIDAB_2881987
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

GORASP2 (Golgi reassembly-stacking protein 2, or GRS2) has multiple phosphorylation sites and can be phosphorylated by MKK/ERK pathway during mitoisis. GORASP2 plays a role in the assembly and membrane stacking of the Golgi cisternae, and in the process by which Golgi stacks reform after mitotic breakdown.GORASP2 is supposed to regulate the intracellular transport and presentation of a defined set of transmembrane proteins, such as transmembrane TGFA.