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  • Featured Product
  • KD/KO Validated

GOSR2/Membrin Polyclonal antibody

GOSR2/Membrin Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 12095-1-AP

Synonyms

Membrin, GS27, GOSR2, Golgi SNAP receptor complex member 2, 27 kDa Golgi SNARE protein

Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using GOSR2/Membrin Polyclonal antibody (12095-1-AP)

    WB analysis of Jurkat using 12095-1-AP

    WB result of GOSR2/Membrin antibody (12095-1-AP; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GOSR2/Membrin transfected Jurkat cells.

  • Western Blot (WB) analysis of various lysates using GOSR2/Membrin Polyclonal antibody (12095-1-AP)

    WB analysis using 12095-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12095-1-AP (GOSR2/Membrin antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using GOSR2/Membrin Polyclonal antibody (12095-1-AP)

    WB analysis of mouse liver using 12095-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 12095-1-AP (GOSR2/Membrin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse stomach tissue using GOSR2/Membrin Polyclonal antibody (12095-1-AP)

    WB analysis of mouse stomach using 12095-1-AP

    mouse stomach tissue were subjected to SDS PAGE followed by western blot with 12095-1-AP (GOSR2/Membrin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human liver tissue using GOSR2/Membrin Polyclonal antibody (12095-1-AP)

    WB analysis of human liver using 12095-1-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 12095-1-AP (GOSR2/Membrin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human stomach tissue using GOSR2/Membrin Polyclonal antibody (12095-1-AP)

    IHC staining of human stomach using 12095-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 12095-1-AP (GOSR2/Membrin antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using GOSR2/Membrin Polyclonal antibody (12095-1-AP)

    IF Staining of HeLa using 12095-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GOSR2/Membrin antibody (12095-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using GOSR2/Membrin Polyclonal antibody (12095-1-AP)

    IF Staining of HepG2 using 12095-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 12095-1-AP (GOSR2/Membrin antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using GOSR2/Membrin Polyclonal antibody (12095-1-AP)

    IF Staining of HepG2 using 12095-1-AP

    Immunofluorescent analysis of () fixed HepG2 cells using 12095-1-AP (GOSR2/Membrin antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

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Tested Applications

Positive WB detected inJurkat cells, human liver tissue, mouse liver tissue, mouse stomach tissue, L02 cells, rat liver tissue
Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells, HepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

12095-1-AP targets GOSR2/Membrin in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GOSR2/Membrin fusion protein Ag2736 相同性解析による交差性が予測される生物種
Full Name golgi SNAP receptor complex member 2
Calculated molecular weight 212 aa, 25 kDa
Observed molecular weight 25 kDa
GenBank accession numberBC009710
Gene symbol Membrin
Gene ID (NCBI) 9570
RRIDAB_2113610
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Golgi SNAP receptor complex member 2 (GOSR2), also known as GS27 or Membrin, is a member of the solube NSF attachment protein receptor (SNARE) family of vesicle docking proteins. GOSR2 is localized in Golgi apparatus. It is involved in transport of proteins from the cis/medial-Golgi to the trans-Golgi network.

Protocols

Product Specific Protocols
WB protocol for GOSR2/Membrin antibody 12095-1-APDownload protocol
IHC protocol for GOSR2/Membrin antibody 12095-1-APDownload protocol
IF protocol for GOSR2/Membrin antibody 12095-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Nat Commun

Lysosomal exocytosis releases pathogenic α-synuclein species from neurons in synucleinopathy models

Authors - Ying Xue Xie
View Article
WB

Commun Biol

Tankyrase-1-mediated degradation of Golgin45 regulates glycosyltransferase trafficking and protein glycosylation in Rab2-GTP-dependent manner.

Authors - Xihua Yue
View Article