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Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using GOSR2/Membrin Monoclonal antibody (66134-1-Ig)

    WB analysis of Jurkat using 66134-1-Ig (same clone as 66134-1-PBS)

    WB result of GOSR2/Membrin antibody (66134-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GOSR2/Membrin transfected Jurkat cells. This data was developed using the same antibody clone with 66134-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using GOSR2/Membrin Monoclonal antibody (66134-1-Ig)

    WB analysis using 66134-1-Ig (same clone as 66134-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66134-1-Ig (GOSR2/Membrin antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66134-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using GOSR2/Membrin Monoclonal antibody (66134-1-Ig)

    WB analysis using 66134-1-Ig (same clone as 66134-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66134-1-Ig (GOSR2/Membrin antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66134-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using GOSR2/Membrin Monoclonal antibody (66134-1-Ig)

    WB analysis of HeLa using 66134-1-Ig (same clone as 66134-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 66134-1-Ig (GOSR2/Membrin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66134-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using GOSR2/Membrin Monoclonal antibody (66134-1-Ig)

    IHC staining of human liver using 66134-1-Ig (same clone as 66134-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver slide using 66134-1-Ig (GOSR2/Membrin antibody at dilution of 1:50. This data was developed using the same antibody clone with 66134-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using GOSR2/Membrin Monoclonal antibody (66134-1-Ig)

    IHC staining of human liver using 66134-1-Ig (same clone as 66134-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver slide using 66134-1-Ig (GOSR2/Membrin antibody at dilution of 1:50. This data was developed using the same antibody clone with 66134-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human liver cancer tissue using GOSR2/Membrin Monoclonal antibody (66134-1-Ig)

    IF Staining of human liver cancer using 66134-1-Ig (same clone as 66134-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using GOSR2/Membrin antibody (66134-1-Ig, Clone: 7E7C7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66134-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

GOSR2/Membrin Monoclonal antibody, PBS Only

GOSR2/Membrin Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 66134-1-PBS
Clone No.7E7C7

Host / Isotype

Mouse / IgG2a

Reactivity

human, rat

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

27 kDa Golgi SNARE protein, Bos1, GOSR2, GS27, Membrin

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66134-1-PBS targets GOSR2/Membrin in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag21393

Product name: Recombinant human GOSR2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-192 aa of BC009710

Sequence: MDPLFQQTHKQVHEIQSCMGRLETADKQSVHIVENEIQASIDQIFSRLERLEILSSKEPPNKRQNARLRVDQLKYDVQHLQTALRNFQHRRHAREQQERQREELLSRTFTTNDSDTTIPMDESLQFNSSLQKVHNGMDDLILDGHNILDGLRTQRLTLKGTQKKILDIANMLGLSNTVMRLIEKRAFQDKYF

相同性解析による交差性が予測される生物種
Full Name golgi SNAP receptor complex member 2
Calculated molecular weight 212 aa, 25 kDa
Observed molecular weight 25 kDa
GenBank accession numberBC009710
Gene Symbol Membrin
Gene ID (NCBI) 9570
RRIDAB_2881533
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDO14653
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Golgi SNAP receptor complex member 2 (GOSR2), also known as GS27 or Membrin, is a member of the solube NSF attachment protein receptor (SNARE) family of vesicle docking proteins. GOSR2 is localized in Golgi apparatus. It is involved in transport of proteins from the cis/medial-Golgi to the trans-Golgi network.

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