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- KD/KO Validated
GOT1 Polyclonal antibody
GOT1 Polyclonal Antibody for WB, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
WB, IF/ICC, IP, ELISA
Conjugate
Unconjugated
Cat no : 14886-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HepG2 cells, L02 cells, mouse brain, rat brain |
| Positive IP detected in | mouse brain tissue |
| Positive IF/ICC detected in | L02 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 4 publications below |
| WB | See 37 publications below |
| IF | See 2 publications below |
| IP | See 1 publications below |
Product Information
14886-1-AP targets GOT1 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | GOT1 fusion protein Ag6671 相同性解析による交差性が予測される生物種 |
| Full Name | glutamic-oxaloacetic transaminase 1, soluble (aspartate aminotransferase 1) |
| Calculated molecular weight | 46 kDa |
| Observed molecular weight | 43-46 kDa |
| GenBank accession number | BC000498 |
| Gene symbol | GOT1 |
| Gene ID (NCBI) | 2805 |
| RRID | AB_2113630 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Glutamate oxaloacetate transaminase 1 (GOT1) catalyzes the reversible reaction of L-aspartate and alpha-ketoglutarate into oxaloacetate and L-glutamate and plays a key role in carbon and nitrogen metabolism. GOT1 can potentially control the intracellular levels of reactive oxygen species (ROS) through NADPH synthesis and enhances tumor growth. GOT1 expression correlates with the growth of several tumors.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for GOT1 antibody 14886-1-AP | Download protocol |
| IF protocol for GOT1 antibody 14886-1-AP | Download protocol |
| IP protocol for GOT1 antibody 14886-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Systematic discovery of mutation-directed neo-protein-protein interactions in cancer. | ||
Cell Metab Rewiring of Glutamine Metabolism Is a Bioenergetic Adaptation of Human Cells with Mitochondrial DNA Mutations. | ||
Cell Res Glutaminase GLS1 senses glutamine availability in a non-enzymatic manner triggering mitochondrial fusion.
| ||
Nat Commun Sirtuin5 contributes to colorectal carcinogenesis by enhancing glutaminolysis in a deglutarylation-dependent manner. | ||
Nat Commun Cooperative STAT/NF-κB signaling regulates lymphoma metabolic reprogramming and aberrant GOT2 expression. | ||
J Exp Med Inhibiting both proline biosynthesis and lipogenesis synergistically suppresses tumor growth. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Daniel (Verified Customer) (04-29-2019) | Strong band of the expected size. Weaker non-specific bands.
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