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  • Featured Product
  • KD/KO Validated

GOT1 Monoclonal antibody, PBS Only

GOT1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, chicken

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

5A12E10

Cat no : 60317-1-PBS

Synonyms

GIG18, EC:2.6.1.1, cCAT, CAT, cAspAT

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GOT1 Monoclonal antibody (60317-1-Ig)

    WB analysis using 60317-1-Ig (same clone as 60317-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60317-1-Ig (GOT1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60317-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using GOT1 Monoclonal antibody (60317-1-Ig)

    WB analysis using 60317-1-Ig (same clone as 60317-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60317-1-Ig (GOT1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60317-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using GOT1 Monoclonal antibody (60317-1-Ig)

    WB analysis using 60317-1-Ig (same clone as 60317-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60317-1-Ig (GOT1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60317-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat heart tissue using GOT1 Monoclonal antibody (60317-1-Ig)

    WB analysis of rat heart using 60317-1-Ig (same clone as 60317-1-PBS)

    rat heart tissue were subjected to SDS PAGE followed by western blot with 60317-1-Ig (GOT1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60317-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using GOT1 Monoclonal antibody (60317-1-Ig)

    IHC staining of human liver cancer using 60317-1-Ig (same clone as 60317-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60317-1-Ig (GOT1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60317-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using GOT1 Monoclonal antibody (60317-1-Ig)

    IHC staining of human liver cancer using 60317-1-Ig (same clone as 60317-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60317-1-Ig (GOT1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60317-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using GOT1 Monoclonal antibody (60317-1-Ig)

    IF Staining of HepG2 using 60317-1-Ig (same clone as 60317-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using GOT1 antibody (60317-1-Ig, Clone: 5A12E10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60317-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60317-1-PBS targets GOT1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat, chicken samples.

Tested Reactivity human, mouse, rat, chicken
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen GOT1 fusion protein Ag7012 相同性解析による交差性が予測される生物種
Full Name glutamic-oxaloacetic transaminase 1, soluble (aspartate aminotransferase 1)
Calculated molecular weight 46 kDa
Observed molecular weight 40-46 kDa
GenBank accession numberBC000498
Gene symbol GOT1
Gene ID (NCBI) 2805
RRIDAB_2881428
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Glutamate oxaloacetate transaminase 1 (GOT1) catalyzes the reversible reaction of L-aspartate and alpha-ketoglutarate into oxaloacetate and L-glutamate and plays a key role in carbon and nitrogen metabolism. GOT1 can potentially control the intracellular levels of reactive oxygen species (ROS) through NADPH synthesis and enhances tumor growth. GOT1 expression correlates with the growth of several tumors.