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KD/KO Validated

GOT2 Polyclonal antibody

Name: GOT2 Polyclonal antibody
Brand: Proteintech
SKU: 14800-1-AP

GOT2 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 14800-1-AP

Synonyms

Fatty acid-binding protein, FABP-1, FABP 1, EC:2.6.1.1, Aspartate aminotransferase, mitochondrial

Validation Data Gallery

Filter:

AllWBIHCIF/ICCFC (Intra)

WB analysis using 14800-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 14800-1-AP (GOT2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
WB analysis of mouse heart using 14800-1-AP
mouse heart tissue were subjected to SDS PAGE followed by western blot with 14800-1-AP (GOT2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse spleen using 14800-1-AP
mouse spleen tissue were subjected to SDS PAGE followed by western blot with 14800-1-AP (GOT2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of A431 using 14800-1-AP
A431 cells were subjected to SDS PAGE followed by western blot with 14800-1-AP (GOT2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 14800-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 14800-1-AP (GOT2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of rat brain using 14800-1-AP
rat brain tissue were subjected to SDS PAGE followed by western blot with 14800-1-AP (GOT2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC staining of human breast cancer using 14800-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14800-1-AP (GOT2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat heart using 14800-1-AP
Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 14800-1-AP (GOT2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat brain using 14800-1-AP
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 14800-1-AP (GOT2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat brain using 14800-1-AP
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 14800-1-AP (GOT2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human skin using 14800-1-AP
Immunohistochemical analysis of paraffin-embedded human skin using 14800-1-AP (GOT2 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human skin using 14800-1-AP
Immunohistochemical analysis of paraffin-embedded human skin using 14800-1-AP (GOT2 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human liver using 14800-1-AP
Immunohistochemical analysis of paraffin-embedded human liver using 14800-1-AP (GOT2 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human liver using 14800-1-AP
Immunohistochemical analysis of paraffin-embedded human liver using 14800-1-AP (GOT2 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human kidney using 14800-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 14800-1-AP (GOT2 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human kidney using 14800-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 14800-1-AP (GOT2 antibody) at dilution of 1:50 (under 40x lens).
IF Staining of MCF-7 using 14800-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using GOT2 antibody (14800-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
FC experiment of HEK-293 using 14800-1-AP
1x10^6 HEK-293 cells were intracellularly stained with 0.25 ug GOT2 Polyclonal antibody (14800-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Cat No. 14800-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	A431 cells, HepG2 cells, mouse heart tissue, mouse spleen tissue, HEK-293 cells, mouse brain, rat brain
Positive IHC detected in	human breast cancer tissue, human skin tissue, rat brain tissue, human liver tissue, rat heart tissue, human kidney tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	MCF-7 cells
Positive FC (Intra) detected in	HEK-293 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:16000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 6 publications below
WB	See 32 publications below
IHC	See 2 publications below
IP	See 1 publications below
CoIP	See 1 publications below

Product Information

14800-1-AP targets GOT2 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	GOT2 fusion protein Ag6514 相同性解析による交差性が予測される生物種
Full Name	glutamic-oxaloacetic transaminase 2, mitochondrial (aspartate aminotransferase 2)
Calculated molecular weight	47 kDa
Observed molecular weight	43-45 kDa
GenBank accession number	BC000525
Gene symbol	GOT2
Gene ID (NCBI)	2806
RRID	AB_2247898
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

GOT2 encodes the mitochondrial glutamate oxaloacetate transaminase and plays an essential role in the intracellular NAD(H) redox balance. Upregulation of GOT2 has been reported in various cancers, including breast cancer and pancreatic cancer. GOT2 mutation leads to autosomal recessive neurometabolic disorder.

Protocols

Product Specific Protocols
WB protocol for GOT2 antibody 14800-1-AP	Download protocol
IHC protocol for GOT2 antibody 14800-1-AP	Download protocol
IF protocol for GOT2 antibody 14800-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Cell Stem Cell Aspartate availability limits hematopoietic stem cell function during hematopoietic regeneration. Authors - Le Qi KO Validated View Article
	WB	Cell Metab Rewiring of Glutamine Metabolism Is a Bioenergetic Adaptation of Human Cells with Mitochondrial DNA Mutations. Authors - Qiuying Chen View Article
human	WB,CoIP	Cell Metab Malic enzyme 2 connects the Krebs cycle intermediate fumarate to mitochondrial biogenesis. Authors - Yi-Ping Wang View Article
mouse	WB	Cell Res Glutaminase GLS1 senses glutamine availability in a non-enzymatic manner triggering mitochondrial fusion. Authors - Wei-Feng Cai KD Validated View Article
human	WB	Nat Commun Sirtuin5 contributes to colorectal carcinogenesis by enhancing glutaminolysis in a deglutarylation-dependent manner. Authors - Yun-Qian Wang View Article
human	WB,IHC	Nat Commun Cooperative STAT/NF-κB signaling regulates lymphoma metabolic reprogramming and aberrant GOT2 expression. Authors - Maren Feist View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GOT2 Polyclonal antibody

GOT2 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Validation Data Gallery

WB analysis using 14800-1-AP

WB analysis of mouse heart using 14800-1-AP

WB analysis of mouse spleen using 14800-1-AP

WB analysis of A431 using 14800-1-AP

WB analysis of HepG2 using 14800-1-AP

WB analysis of rat brain using 14800-1-AP

IHC staining of human breast cancer using 14800-1-AP

IHC staining of rat heart using 14800-1-AP

IHC staining of rat brain using 14800-1-AP

IHC staining of rat brain using 14800-1-AP

IHC staining of human skin using 14800-1-AP

IHC staining of human skin using 14800-1-AP

IHC staining of human liver using 14800-1-AP

IHC staining of human liver using 14800-1-AP

IHC staining of human kidney using 14800-1-AP

IHC staining of human kidney using 14800-1-AP

IF Staining of MCF-7 using 14800-1-AP

FC experiment of HEK-293 using 14800-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Aspartate availability limits hematopoietic stem cell function during hematopoietic regeneration.

Rewiring of Glutamine Metabolism Is a Bioenergetic Adaptation of Human Cells with Mitochondrial DNA Mutations.

Malic enzyme 2 connects the Krebs cycle intermediate fumarate to mitochondrial biogenesis.

Glutaminase GLS1 senses glutamine availability in a non-enzymatic manner triggering mitochondrial fusion.

Sirtuin5 contributes to colorectal carcinogenesis by enhancing glutaminolysis in a deglutarylation-dependent manner.

Cooperative STAT/NF-κB signaling regulates lymphoma metabolic reprogramming and aberrant GOT2 expression.

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