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  • Featured Product
  • KD/KO Validated

GP73/GOLPH2 Monoclonal antibody, PBS Only

GP73/GOLPH2 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

3D1F1

Cat no : 66331-1-PBS

Synonyms

bA379P1.3, C9orf155, FLJ22634, FLJ23608, Golgi marker, golgi membrane protein 1, Golgi membrane protein GP73, Golgi phosphoprotein 2, GOLM1, GOLPH2, GP73, GP73/GOLPH2, PSEC0257, UNQ686/PRO1326

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GP73/GOLPH2 Monoclonal antibody (66331-1-Ig)

    WB analysis using 66331-1-Ig (same clone as 66331-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66331-1-Ig (GP73/GOLPH2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66331-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using GP73/GOLPH2 Monoclonal antibody (66331-1-Ig)

    IHC staining of human liver cancer using 66331-1-Ig (same clone as 66331-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66331-1-Ig (GP73/GOLPH2 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66331-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using GP73/GOLPH2 Monoclonal antibody (66331-1-Ig)

    IHC staining of human liver cancer using 66331-1-Ig (same clone as 66331-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66331-1-Ig (GP73/GOLPH2 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66331-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using GP73/GOLPH2 Monoclonal antibody (66331-1-Ig)

    IF Staining of HeLa using 66331-1-Ig (same clone as 66331-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GP73/GOLPH2 antibody (66331-1-Ig, Clone: 3D1F1 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66331-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66331-1-PBS targets GP73/GOLPH2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen GP73/GOLPH2 fusion protein Ag7207 相同性解析による交差性が予測される生物種
Full Name golgi membrane protein 1
Calculated molecular weight 45 kDa
Observed molecular weight 73 kDa
GenBank accession numberBC001740
Gene symbol GP73
Gene ID (NCBI) 51280
RRIDAB_2881712
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS only
Storage ConditionsStore at -80°C.

Background Information

Golgi phosphoprotein 2 (GOLPH2, also known as GP73 or GOLM1) is a resident Golgi type-II membrane protein. It is predominantly expressed in the epithelial cells of many human tissues. GOLPH2 traffics through endosomes and can be secreted into the circulation. Its expression is upregulated in a number of tumors and GOLPH2 could be a promising serum marker for hepatocellular carcinoma. We got 73 kDa in western blotting due to phosphorylation.