Validation Data Gallery
Tested Applications
Recommended dilution
| Application | Dilution |
|---|---|
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Product Information
66331-1-PBS targets GP73/GOLPH2 as part of a matched antibody pair:
MP51312-3: 66331-2-PBS capture and 66331-1-PBS detection (validated in Cytometric bead array)
MP51312-4: 66331-3-PBS capture and 66331-1-PBS detection (validated in Cytometric bead array)
Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.
This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.
| Tested Reactivity | human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | GP73/GOLPH2 fusion protein Ag7207 相同性解析による交差性が予測される生物種 |
| Full Name | golgi membrane protein 1 |
| Calculated molecular weight | 45 kDa |
| Observed molecular weight | 73 kDa |
| GenBank accession number | BC001740 |
| Gene Symbol | GP73 |
| Gene ID (NCBI) | 51280 |
| RRID | AB_2881712 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q8NBJ4 |
| Storage Buffer | PBS Only |
| Storage Conditions | Store at -80°C. |
Background Information
Golgi phosphoprotein 2 (GOLPH2, also known as GP73 or GOLM1) is a resident Golgi type-II membrane protein. It is predominantly expressed in the epithelial cells of many human tissues. GOLPH2 traffics through endosomes and can be secreted into the circulation. Its expression is upregulated in a number of tumors and GOLPH2 could be a promising serum marker for hepatocellular carcinoma. We got 73 kDa in western blotting due to phosphorylation.