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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GP73/GOLPH2 Recombinant antibody (81893-1-RR)

    WB analysis using 81893-1-RR (same clone as 81893-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 81893-1-RR (GP73/GOLPH2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81893-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovarian cancer using GP73/GOLPH2 Recombinant antibody (81893-1-RR)

    IHC staining of human ovarian cancer using 81893-1-RR (same clone as 81893-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 81893-1-RR (GP73/GOLPH2 antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81893-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using GP73/GOLPH2 Recombinant antibody (81893-1-RR)

    IHC staining of human liver cancer using 81893-1-RR (same clone as 81893-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 81893-1-RR (GP73/GOLPH2 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81893-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using GP73/GOLPH2 Recombinant antibody (81893-1-RR)

    IHC staining of human liver cancer using 81893-1-RR (same clone as 81893-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 81893-1-RR (GP73/GOLPH2 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81893-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach tissue using GP73/GOLPH2 Recombinant antibody (81893-1-RR)

    IHC staining of human stomach using 81893-1-RR (same clone as 81893-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 81893-1-RR (GP73/GOLPH2 antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81893-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using GP73/GOLPH2 Recombinant antibody (81893-1-RR)

    IHC staining of human stomach cancer using 81893-1-RR (same clone as 81893-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 81893-1-RR (GP73/GOLPH2 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81893-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using GP73/GOLPH2 Recombinant antibody (81893-1-RR)

    IHC staining of human stomach cancer using 81893-1-RR (same clone as 81893-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 81893-1-RR (GP73/GOLPH2 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81893-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using GP73/GOLPH2 Recombinant antibody (81893-1-RR)

    IF Staining of HeLa using 81893-1-RR (same clone as 81893-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GP73/GOLPH2 antibody (81893-1-RR, Clone: 1I12 ) at dilution of 1:450 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 81893-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using GP73/GOLPH2 Recombinant antibody (81893-1-RR)

    IF Staining of HeLa using 81893-1-RR (same clone as 81893-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GP73/GOLPH2 antibody (81893-1-RR, Clone: 1I12 ) at dilution of 1:900 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 81893-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 81893-1-RR

    Affinity and Kinetic Characterization of 81893-1-RR

    Biolayer interferometry (BLl) kinetic assays of 81893-1-RR against Human GP73/GOLPH2 were performed. The affinity constant is 0.828 nM.

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GP73/GOLPH2 Recombinant antibody, PBS Only

GP73/GOLPH2 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 81893-1-PBS
Clone No.1I12

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

GOLM1, GOLPH2, GP73, 1I12, C9orf155

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

81893-1-PBS targets GP73/GOLPH2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen GP73/GOLPH2 fusion protein Ag7207 相同性解析による交差性が予測される生物種
Full Name golgi membrane protein 1
Calculated molecular weight 45 kDa
Observed molecular weight70-75 kDa
GenBank accession numberBC001740
Gene Symbol GP73
Gene ID (NCBI) 51280
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ8NBJ4
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Golgi phosphoprotein 2 (GOLPH2, also known as GP73 or GOLM1) is a resident Golgi type-II membrane protein. It is predominantly expressed in the epithelial cells of many human tissues. GOLPH2 traffics through endosomes and can be secreted into the circulation. Its expression is upregulated in a number of tumors and GOLPH2 could be a promising serum marker for hepatocellular carcinoma. We got 70-75 kDa in western blotting due to phosphorylation.