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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CD42a / GP9 Monoclonal antibody

CD42a / GP9 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2D4H1

Cat no : 67271-1-Ig

Synonyms

CD42a, Glycoprotein 9, glycoprotein IX (platelet), GP IX, GP9, GPIX, Platelet glycoprotein IX

Validation Data Gallery

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  • Western Blot (WB) analysis of human peripheral blood platelets using CD42a / GP9 Monoclonal antibody (67271-1-Ig)

    WB analysis of human peripheral blood platelets using 67271-1-Ig

    Human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 67271-1-Ig (CD42a / GP9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human spleen tissue using CD42a / GP9 Monoclonal antibody (67271-1-Ig)

    IHC staining of human spleen using 67271-1-Ig

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 67271-1-Ig (CD42a / GP9 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human spleen tissue using CD42a / GP9 Monoclonal antibody (67271-1-Ig)

    IHC staining of human spleen using 67271-1-Ig

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 67271-1-Ig (CD42a / GP9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inHuman peripheral blood platelets tissue
Positive IHC detected inhuman spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67271-1-Ig targets CD42a / GP9 in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen CD42a / GP9 fusion protein Ag6132 相同性解析による交差性が予測される生物種
Full Name glycoprotein IX (platelet)
Calculated molecular weight 19 kDa
Observed molecular weight 20 kDa
GenBank accession numberBC030229
Gene symbol CD42a
Gene ID (NCBI) 2815
RRIDAB_2882540
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Platelet glycoprotein IX (GP9, also known as CD42a) is a relatively small surface glycoprotein present on platelets and megakaryocytes. GP9 forms a 1:1 non-covalent complex with GPIb (CD42b). The GPIb-V-IX complex functions as the vWF receptor and mediates vWF-dependent platelet adhesion to blood vessels. Defects in GP9 are a cause of Bernard-Soulier syndrome, also known as giant platelet disease.

Protocols

Product Specific Protocols
WB protocol for CD42a / GP9 antibody 67271-1-IgDownload protocol
IHC protocol for CD42a / GP9 antibody 67271-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols