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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GPAM Polyclonal antibody, PBS Only

GPAM Polyclonal Antibody for IHC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

IHC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 12454-1-PBS

Synonyms

Validation Data Gallery

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  • Immunoprecipitation (IP) experiment of mouse liver tissue using GPAM Polyclonal antibody (12454-1-AP)

    IP experiment of mouse liver using 12454-1-AP (same clone as 12454-1-PBS)

    IP result of anti-GPAM (IP:12454-1-AP, 4ug; Detection:12454-1-AP 1:2000) with mouse liver tissue lysate 1840 ug. This data was developed using the same antibody clone with 12454-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat brain tissue using GPAM Polyclonal antibody (12454-1-AP)

    IHC staining of rat brain using 12454-1-AP (same clone as 12454-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 12454-1-AP (GPAM antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12454-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat brain tissue using GPAM Polyclonal antibody (12454-1-AP)

    IHC staining of rat brain using 12454-1-AP (same clone as 12454-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 12454-1-AP (GPAM antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12454-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

12454-1-PBS targets GPAM in IHC, IP, Indirect ELISA applications and shows reactivity with mouse samples.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GPAM fusion protein Ag3120 相同性解析による交差性が予測される生物種
Full Name glycerol-3-phosphate acyltransferase, mitochondrial
Calculated molecular weight 828 aa, 94 kDa
Observed molecular weight90-92 kDa
GenBank accession numberBC030783
Gene symbol GPAM
Gene ID (NCBI) 57678
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

GPAM (Glycerol-3-Phosphate Acyltransferase, Mitochondrial) is a Protein Coding gene. This gene encodes a mitochondrial enzyme which prefers saturated fatty acids as its substrate for the synthesis of glycerolipids. This metabolic pathway's first step is catalyzed by the encoded enzyme. Two forms for this enzyme exist, one in the mitochondria and one in the endoplasmic reticulum. Two alternatively spliced transcript variants have been described for this gene.Diseases associated with GPAM include Rhizomelic Chondrodysplasia Punctata, Type 2 and Congenital Generalized Lipodystrophy. GPAM can be detected as 90-92 kDa.