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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GPATCH2 Polyclonal antibody

GPATCH2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 24366-1-AP

Synonyms

G patch domain containing 2, GPATC2, GPATCH2, RP11 361K17.1

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse spleen tissue using GPATCH2 Polyclonal antibody (24366-1-AP)

    WB analysis of mouse spleen using 24366-1-AP

    mouse spleen tissue were subjected to SDS PAGE followed by western blot with 24366-1-AP (GPATCH2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse thymus tissue using GPATCH2 Polyclonal antibody (24366-1-AP)

    WB analysis of mouse thymus using 24366-1-AP

    mouse thymus tissue were subjected to SDS PAGE followed by western blot with 24366-1-AP (GPATCH2 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

  • Western Blot (WB) analysis of mouse kidney tissue using GPATCH2 Polyclonal antibody (24366-1-AP)

    WB analysis of mouse kidney using 24366-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 24366-1-AP (GPATCH2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using GPATCH2 Polyclonal antibody (24366-1-AP)

    IHC staining of human breast cancer using 24366-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 24366-1-AP (GPATCH2 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using GPATCH2 Polyclonal antibody (24366-1-AP)

    IHC staining of human testis using 24366-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24366-1-AP (GPATCH2 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using GPATCH2 Polyclonal antibody (24366-1-AP)

    IHC staining of human lung cancer using 24366-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 24366-1-AP (GPATCH2 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using GPATCH2 Polyclonal antibody (24366-1-AP)

    IHC staining of human lung cancer using 24366-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 24366-1-AP (GPATCH2 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using GPATCH2 Polyclonal antibody (24366-1-AP)

    IHC staining of human testis using 24366-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24366-1-AP (GPATCH2 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using GPATCH2 Polyclonal antibody (24366-1-AP)

    IHC staining of human breast cancer using 24366-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 24366-1-AP (GPATCH2 Antibody) at dilution of 1:50 (under 10x lens).

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Tested Applications

Positive WB detected inmouse spleen tissue, mouse kidney tissue, mouse thymus tissue
Positive IHC detected inhuman breast cancer tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

The immunogen of 24366-1-AP is GPATCH2 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivity mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GPATCH2 fusion protein Ag21647 相同性解析による交差性が予測される生物種
Full Name G patch domain containing 2
Calculated molecular weight 528 aa, 59 kDa
Observed molecular weight 65-70 kDa
GenBank accession numberBC063474
Gene symbol GPATCH2
Gene ID (NCBI) 55105
RRIDAB_2879508
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for GPATCH2 antibody 24366-1-APDownload protocol
IHC protocol for GPATCH2 antibody 24366-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Cell Death Dis

Deletion of Gpatch2 does not alter Tnf expression in mice

Authors - Destiny Dalseno
View Article