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  • Featured Product
  • KD/KO Validated

GPBP1 Polyclonal antibody

GPBP1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 21622-1-AP

Synonyms

GPBP, GPBP1, SSH6, Vascular wall linked protein, VASCULIN

Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using GPBP1 Polyclonal antibody (21622-1-AP)

    WB analysis of HepG2 using 21622-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 21622-1-AP (GPBP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of L02 cells using GPBP1 Polyclonal antibody (21622-1-AP)

    WB analysis of L02 using 21622-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 21622-1-AP (GPBP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human small intestine tissue using GPBP1 Polyclonal antibody (21622-1-AP)

    IHC staining of human small intestine using 21622-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 21622-1-AP (GPBP1 antibody) at dilution of 1:300 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human small intestine tissue using GPBP1 Polyclonal antibody (21622-1-AP)

    IHC staining of human small intestine using 21622-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 21622-1-AP (GPBP1 antibody) at dilution of 1:300 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human small intestine tissue using GPBP1 Polyclonal antibody (21622-1-AP)

    IHC staining of human small intestine using 21622-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine slide using 21622-1-AP (GPBP1 Antibody) at dilution of 1:50.

  • Immunohistochemistry (IHC) staining of human testis tissue using GPBP1 Polyclonal antibody (21622-1-AP)

    IHC staining of human testis using 21622-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis slide using 21622-1-AP (GPBP1 Antibody) at dilution of 1:50.

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Tested Applications

Positive WB detected inHepG2 cells, L02 cells
Positive IHC detected inhuman small intestine tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:150-1:600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

21622-1-AP targets GPBP1 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GPBP1 fusion protein Ag14877 相同性解析による交差性が予測される生物種
Full Name GC-rich promoter binding protein 1
Calculated molecular weight 473 aa, 53 kDa
Observed molecular weight 45 kDa
GenBank accession numberBC000267
Gene symbol GPBP1
Gene ID (NCBI) 65056
RRIDAB_10732822
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for GPBP1 antibody 21622-1-APDownload protocol
IHC protocol for GPBP1 antibody 21622-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IP

Exp Mol Med

Loss of RTN3 phenocopies chronic kidney disease and results in activation of the IGF2-JAK2 pathway in proximal tubular epithelial cells.

Authors - Liang-Liang Fan
  • KD Validated
View Article