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Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using GPC1 Recombinant antibody (83318-4-RR)

    WB analysis of HepG2 using 83318-4-RR

    HepG2 cells were subjected to SDS PAGE followed by western blot with 83318-4-RR (GPC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 83318-4-RR

    Affinity and Kinetic Characterization of 83318-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83318-4-RR against Human GPC1 were performed. The affinity constant is 3.58 nM.

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GPC1 Recombinant antibody

GPC1 Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 83318-4-RR
Clone No.240207G5

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Synonyms

glypican 1, Glypican-1, glypican1, glypican, FLJ38078

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive WB detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83318-4-RR targets GPC1 in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein 相同性解析による交差性が予測される生物種
Full Name glypican 1
Calculated molecular weight 558 aa, 62 kDa
Observed molecular weight 61-65 kDa
GenBank accession numberBC051279
Gene Symbol GPC1
Gene ID (NCBI) 2817
RRIDAB_3670984
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP35052
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Glypicans are a family of HSPGs that are attached to the cell membrane by a glycosyl-phosphatidylinositol anchor. Glypicans are considered to have the ability to modulate the activities of HBGFs. The expression of glypicans is regulated temporally and spatially during development, suggesting they are involved in development and morphogenesis. To date, six glypicans (GPC1 to GPC6) have been identified in mammals. GPC1 is most ubiquitously expressed in adult tissues. GPC1 promotes efficient signaling by HBGFs and plays a critical role in cell growth and differentiation. GPC1 is involved in tumorigenesis and angiogenesis, and is frequently overexpressed in several types of tumors including pancreatic carcinoma, breast cancer and glioma.

Protocols

Product Specific Protocols
WB protocol for GPC1 antibody 83318-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols