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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GPC3 Polyclonal antibody, PBS Only

GPC3 Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 30021-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GPC3 Polyclonal antibody (30021-1-AP)

    WB analysis using 30021-1-AP (same clone as 30021-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30021-1-AP (GPC3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30021-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using GPC3 Polyclonal antibody (30021-1-AP)

    IHC staining of mouse liver using 30021-1-AP (same clone as 30021-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 30021-1-AP (GPC3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30021-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

30021-1-PBS targets GPC3 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GPC3 fusion protein Ag32559 相同性解析による交差性が予測される生物種
Full Name glypican 3
Calculated molecular weight 580 aa, 66 kDa
Observed molecular weight66 kDa
GenBank accession numberBC035972
Gene symbol GPC3
Gene ID (NCBI) 2719
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Glypicans (GPCs) are a family of glycosylphosphatidylinositol (GPI)-anchored heparan sulfate proteoglycans (HSPGs) that may play a role in the control of cell division and growth regulation. In mammals, there are six GPCs (GPC1 to GPC6), all of which have a similar core-protein size of approx. 60 kDa and the clustering of glycosaminoglycan attachment site near the C-terminus. They are tethered to the cell surface by GPI linkages, which can be cleaved by endogenous phospholipases, thus releasing the protein. Glypican 3 (GPC3) is highly expressed in many tissues during development and plays an important role in the regulation of embryonic growth (PMID: 22467855). Loss-of-function mutations of GPC3 result in the Simpson-Golabi-Behmel overgrowth syndrome (SGBS), and Gpc-3 null mice display developmental overgrowth (PMID: 8589713; 18477453). In hepatocellular carcinoma (HCC), the overexpression of glypican 3 has been demonstrated to be a reliable diagnostic indicator (PMID: 19212669; 22706665). The calculated molecular weight of native glypican 3 is 66 kDa, and glycinate forms of glypican 3 have higher molecular weights than 66 kDa (PMID: 12851874; 16024626; 19574424).