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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GPD1 Polyclonal antibody (13451-1-AP)

    WB analysis using 13451-1-AP (same clone as 13451-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 13451-1-AP (GPD1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13451-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using GPD1 Polyclonal antibody (13451-1-AP)

    WB analysis of HepG2 using 13451-1-AP (same clone as 13451-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 13451-1-AP (GPD1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13451-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse heart tissue using GPD1 Polyclonal antibody (13451-1-AP)

    WB analysis of mouse heart using 13451-1-AP (same clone as 13451-1-PBS)

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 13451-1-AP (GPD1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13451-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using GPD1 Polyclonal antibody (13451-1-AP)

    IF Staining of HepG2 using 13451-1-AP (same clone as 13451-1-PBS)

    Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 13451-1-AP (GPD1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13451-1-PBS in a different storage buffer formulation.

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GPD1 Polyclonal antibody, PBS Only

GPD1 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 13451-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

GPDC, GPD C, GPD 1, Glycerol-3-phosphate dehydrogenase [NAD(+)], cytoplasmic, EC:1.1.1.8

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

13451-1-PBS targets GPD1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GPD1 fusion protein Ag4278 相同性解析による交差性が予測される生物種
Full Name glycerol-3-phosphate dehydrogenase 1 (soluble)
Calculated molecular weight 349 aa, 38 kDa
Observed molecular weight 32-42 kDa
GenBank accession numberBC032234
Gene Symbol GPD1
Gene ID (NCBI) 2819
RRIDAB_10646471
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP21695
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

GPD1 (Glycerol-3-phosphate dehydrogenase 1) is an important enzyme belonging to the NAD-dependent glycerol-3-phosphate dehydrogenase family. Its C-terminal structural domain contains multiple helical structures for binding the substrate DHAP, and its N-terminal structural domain contains a β-folded core for binding NADH. GPD1 catalyzes the conversion of dihydroxyacetone phosphate (DHAP) and reduced nicotinamide adenine dinucleotide (NADH) to glycerol-3-phosphate (G3P) and NAD+, and plays a key role in carbohydrate and lipid metabolism. GPD1 also works with mitochondrial glycerol-3-phosphate dehydrogenase to form a glycerophosphate shuttle system that facilitates the transfer of reducing equivalents from the cytoplasm to the mitochondria. Abnormal activity of GPD1 has been associated with a variety of metabolic disorders, such as obesity, hypertriglyceridemia, and GPD1 has been implicated in cancer, potentially acting as a tumor suppressor.