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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GPD1 Recombinant antibody

GPD1 Recombinant Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

242359D3

Cat no : 84800-1-RR

Synonyms

GPD C, GPD 1, Glycerol-3-phosphate dehydrogenase [NAD(+)], cytoplasmic, EC:1.1.1.8, cGPDH

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GPD1 Recombinant antibody (84800-1-RR)

    WB analysis using 84800-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84800-1-RR (GPD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using GPD1 Recombinant antibody (84800-1-RR)

    IF Staining of HepG2 using 84800-1-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using GPD1 antibody (84800-1-RR, Clone: 242359D3 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 84800-1-RR

    Affinity and Kinetic Characterization of 84800-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84800-1-RR against Human GPD1 were performed. The affinity constant is below 1 pM.

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Tested Applications

Positive WB detected inHepG2 cells, mouse liver tissue
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84800-1-RR targets GPD1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen GPD1 fusion protein Ag4278 相同性解析による交差性が予測される生物種
Full Name glycerol-3-phosphate dehydrogenase 1 (soluble)
Calculated molecular weight 349 aa, 38 kDa
Observed molecular weight32 kDa
GenBank accession numberBC032234
Gene symbol GPD1
Gene ID (NCBI) 2819
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GPD1 (Glycerol-3-phosphate dehydrogenase 1) is an important enzyme belonging to the NAD-dependent glycerol-3-phosphate dehydrogenase family. Its C-terminal structural domain contains multiple helical structures for binding the substrate DHAP, and its N-terminal structural domain contains a β-folded core for binding NADH. GPD1 catalyzes the conversion of dihydroxyacetone phosphate (DHAP) and reduced nicotinamide adenine dinucleotide (NADH) to glycerol-3-phosphate (G3P) and NAD+, and plays a key role in carbohydrate and lipid metabolism. GPD1 also works with mitochondrial glycerol-3-phosphate dehydrogenase to form a glycerophosphate shuttle system that facilitates the transfer of reducing equivalents from the cytoplasm to the mitochondria. Abnormal activity of GPD1 has been associated with a variety of metabolic disorders, such as obesity, hypertriglyceridemia, and GPD1 has been implicated in cancer, potentially acting as a tumor suppressor.

Protocols

Product Specific Protocols
WB protocol for GPD1 antibody 84800-1-RRDownload protocol
IF protocol for GPD1 antibody 84800-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols