Validation Data Gallery
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Tested Applications
| Positive WB detected in | mouse heart tissue, human skeletal muscle tissue, human brain tissue, HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 7 publications below |
Product Information
17263-1-AP targets GPD1L in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | GPD1L fusion protein Ag10977 相同性解析による交差性が予測される生物種 |
| Full Name | glycerol-3-phosphate dehydrogenase 1-like |
| Calculated molecular weight | 351 aa, 38 kDa |
| Observed molecular weight | 38-40 kDa |
| GenBank accession number | BC028726 |
| Gene Symbol | GPD1L |
| Gene ID (NCBI) | 23171 |
| RRID | AB_2112359 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8N335 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Glycerol-3-phosphate dehydrogenase 1-like (GPD1L) is encoded by the gene GPD1L, which is located on chromosome 3p22.3. GPD1L is responsible for catalyzing the conversion of sn-glycerol 3-phosphate to glycerone phosphate. GPD1L displayed a dysregulated state in a wide variety of cancers.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for GPD1L antibody 17263-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Metab Glycerol-3-phosphate biosynthesis regenerates cytosolic NAD+ to alleviate mitochondrial disease. | ||
Proc Natl Acad Sci U S A A ferroptosis defense mechanism mediated by glycerol-3-phosphate dehydrogenase 2 in mitochondria. | ||
J Transl Med Atrial myocyte-derived exosomal microRNA contributes to atrial fibrosis in atrial fibrillation | ||
Cell Death Dis A miR-210-3p regulon that controls the Warburg effect by modulating HIF-1α and p53 activity in triple-negative breast cancer. | ||
Mol Cell Uncoupled glycerol-3-phosphate shuttle in kidney cancer reveals that cytosolic GPD is essential to support lipid synthesis
| ||
Nat Cell Biol AMPK-regulated glycerol excretion maintains metabolic crosstalk between reductive and energetic stress |